Bainbridge G, Gokce I, Lakey J H
Department of Biochemistry and Genetics, The Medical School, University of Newcastle Upon Tyne, UK.
FEBS Lett. 1998 Jul 24;431(3):305-8. doi: 10.1016/s0014-5793(98)00761-3.
Beta-barrel pores are found in outer membrane porins of gram-negative bacteria, bacterial toxins and mitochondrial channels. Apart from the beta-barrel the three groups show no close sequence or structural homology but these pores exhibit symmetrical voltage gating when reconstituted into planar lipid bilayers. The structures of several of these are known and many site-directed mutants have been examined. As a result it seems evident that the gating is a common characteristic of these unrelated large pores and is not generated by specialised structures in the pore lumen.
β-桶状孔道存在于革兰氏阴性菌的外膜孔蛋白、细菌毒素和线粒体通道中。除了β-桶状结构外，这三类物质在序列或结构上没有紧密的同源性，但当这些孔道重组到平面脂质双分子层中时，它们会表现出对称的电压门控特性。其中几种孔道的结构已为人所知，并且已经检测了许多定点突变体。因此，很明显，门控是这些不相关的大孔道的共同特征，并非由孔腔内的特殊结构产生。
