单子叶植物中一个编码来自大麦（Hordeum vulgare）的二腺苷5'，5'''-P1，P4-四磷酸水解酶的新cDNA的克隆与表达

Cloning and expression of a new cDNA from monocotyledonous plants coding for a diadenosine 5',5'''-P1,P4-tetraphosphate hydrolase from barley (Hordeum vulgare).

作者信息

Churin J, Hause B, Feussner I, Maucher H P, Feussner K, Börner T, Wasternack C

机构信息

Institute of Biology, Division of Genetics, Humboldt-University Berlin, Germany.

出版信息

FEBS Lett. 1998 Jul 24;431(3):481-5. doi: 10.1016/s0014-5793(98)00819-9.

DOI:10.1016/s0014-5793(98)00819-9

PMID:9714569

Abstract

From a cDNA library generated from mRNA of white leaf tissues of the ribosome-deficient mutant 'albostrians' of barley (Hordeum vulgare cv. Haisa) a cDNA was isolated carrying 54.2% identity to a recently published cDNA which codes for the diadenosine-5',5'''-P1,P4-tetraphosphate (Ap4A) hydrolase of Lupinus angustifolius (Maksel et al. (1998) Biochem. J. 329, 313-319), and 69% identity to four partial peptide sequences of Ap4A hydrolase of tomato. Overexpression in Escherichia coli revealed a protein of about 19 kDa, which exhibited Ap4A hydrolase activity and cross-reactivity with an antibody raised against a purified tomato Ap4A hydrolase (Feussner et al. (1996) Z. Naturforsch. 51c, 477-486). Expression studies showed an mRNA accumulation in all organs of a barley seedling. Possible functions of Ap4A hydrolase in plants will be discussed.

摘要

从大麦（Hordeum vulgare cv. Haisa）核糖体缺陷型突变体‘albostrians’白叶组织的mRNA构建的cDNA文库中，分离出一个cDNA，它与最近发表的编码羽扇豆（Lupinus angustifolius）二腺苷-5',5'''-P1,P4-四磷酸（Ap4A）水解酶的cDNA有54.2%的同源性（Maksel等人，（1998年）《生物化学杂志》329卷，313 - 319页），与番茄Ap4A水解酶的四个部分肽序列有69%的同源性。在大肠杆菌中的过表达揭示了一种约19 kDa的蛋白质，它具有Ap4A水解酶活性，并与针对纯化的番茄Ap4A水解酶产生的抗体发生交叉反应（Feussner等人，（1996年）《自然科学杂志》51c卷，477 - 486页）。表达研究表明，大麦幼苗的所有器官中都有mRNA积累。将讨论Ap4A水解酶在植物中的可能功能。