活细胞的数字成像显微镜技术。
Digital imaging microscopy of living cells.
作者信息
Rizzuto R, Carrington W, Tuft R A
机构信息
Dept of Biomedical Sciences, University of Padova, Italy.
出版信息
Trends Cell Biol. 1998 Jul;8(7):288-92. doi: 10.1016/s0962-8924(98)01301-4.
PMID:9714601
Abstract
Fluorescence microscopy has undergone a resurgence in interest following the discovery of green-fluorescent protein (GFP) and its increasing use in live-cell imaging. This article describes an enhanced form of epifluorescence microscopy, digital imaging microscopy, that can be used to produce high-resolution three-dimensional images of samples labelled with GFP, or other fluorochromes, using simple instrumentation and image-restoration software.
摘要
随着绿色荧光蛋白（GFP）的发现及其在活细胞成像中的广泛应用，荧光显微镜重新引起了人们的关注。本文介绍了一种增强型落射荧光显微镜——数字成像显微镜，它可利用简单的仪器设备和图像恢复软件，对用GFP或其他荧光染料标记的样品生成高分辨率三维图像。
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