Kong L, Ogawa N, McGuff H S, Nakabayashi T, Sakata K M, Masago R, Vela-Roch N, Talal N, Dang H
Division of Clinical Immunology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, 78284, USA.
Clin Immunol Immunopathol. 1998 Aug;88(2):133-41. doi: 10.1006/clin.1998.4556.
To investigate the molecular mechanism of glandular parenchyma destruction in Sjögren's syndrome (SS), Bcl-2, Bax, Bcl-X, and Bak expression were studied. SS (n = 18) and control salivary glands (n = 6) were examined by immunohistochemistry. Apoptosis was assessed by in situ DNA nick end labeling. Infiltrating mononuclear cells in the SS salivary gland showed elevated Bcl-2. These mononuclear cells expressed increased Bax but did not undergo apoptosis. Both SS and control salivary gland ductal epithelial cells expressed Bcl-2, Bax, and Bcl-X. SS, but not normal, salivary gland acinar cells expressed Bax and underwent apoptosis. These results suggest that elevated Bax expression in SS salivary gland acinar cells may play an important role in the apoptotic pathway. In contrast, Bcl-2 expression in SS infiltrating mononuclear cells and ductal cells may contribute to their survival.
为研究干燥综合征（SS）中腺实质破坏的分子机制，对Bcl-2、Bax、Bcl-X和Bak的表达进行了研究。采用免疫组织化学方法检测了18例SS患者和6例对照唾液腺。通过原位DNA缺口末端标记评估细胞凋亡。SS唾液腺中的浸润单核细胞显示Bcl-2升高。这些单核细胞Bax表达增加但未发生凋亡。SS和对照唾液腺导管上皮细胞均表达Bcl-2、Bax和Bcl-X。SS唾液腺腺泡细胞而非正常腺泡细胞表达Bax并发生凋亡。这些结果表明，SS唾液腺腺泡细胞中Bax表达升高可能在凋亡途径中起重要作用。相反，SS浸润单核细胞和导管细胞中Bcl-2的表达可能有助于它们的存活。
