Lowe M, Kreis T E
Department of Cell Biology, Sciences III, University of Geneva, 30 quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland.
Biochim Biophys Acta. 1998 Aug 14;1404(1-2):53-66. doi: 10.1016/s0167-4889(98)00046-9.
Intracellular membrane transport is mediated predominantly by vesicles which bud from one compartment and fuse specifically with the next compartment in the pathway, resulting in delivery of cargo. COPI-coated vesicles were first identified as intermediates in intra-Golgi transport and subsequent work has shown that they are also involved in transport between the endoplasmic reticulum and the Golgi complex. The COPI coat components have been characterised in detail at the molecular level and a role for membrane proteins and lipids in membrane recruitment of COPI has been uncovered. However, precisely how these distinct membrane components regulate coat recruitment is still unclear and is currently a matter for debate. Furthermore, it is still not clear at exactly how many transport steps COPI is involved and whether it mediates secretory transport in the anterograde or retrograde direction or both. This review focuses on our understanding of COPI structure and function and describes recent findings on the sites of action of COPI in animal cells.
细胞内膜运输主要由囊泡介导，这些囊泡从一个区室出芽，并在运输途径中与下一个区室特异性融合，从而实现货物的递送。COP I被膜小泡最初被鉴定为高尔基体内运输的中间体，随后的研究表明它们也参与内质网和高尔基复合体之间的运输。COP I被膜成分已在分子水平上得到详细表征，并且已经揭示了膜蛋白和脂质在COP I膜募集中的作用。然而，这些不同的膜成分究竟如何调节衣被募集仍不清楚，目前仍是一个有争议的问题。此外，目前仍不清楚COP I具体参与了多少运输步骤，以及它是介导顺行还是逆行方向的分泌运输，还是两者都介导。本综述重点介绍了我们对COP I结构和功能的理解，并描述了动物细胞中COP I作用位点的最新发现。
