人类水通道蛋白7基因的分子特征及其染色体定位

Molecular characterization of human Aquaporin-7 gene and its chromosomal mapping.

作者信息

Ishibashi K, Yamauchi K, Kageyama Y, Saito-Ohara F, Ikeuchi T, Marumo F, Sasaki S

机构信息

Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.

出版信息

Biochim Biophys Acta. 1998 Jul 30;1399(1):62-6. doi: 10.1016/s0167-4781(98)00094-3.

DOI:10.1016/s0167-4781(98)00094-3

PMID:9714739

Abstract

The cDNA for the seventh mammalian aquaporin (AQP7) was isolated from rat testis, and its expression demonstrated at the tail of late spermatids (Ishibashi et al., J. Biol. Chem. 272 (1997) 20,782-20,786). Here we report the isolation of the mouse and the human AQP7 cDNA and the human AQP7 gene. The human AQP7 gene is identical with human adipose AQP (AQPap or AQP7L). The deduced amino acid sequences of human and mouse AQP7 were 68% and 79% identical to those of rat AQP7, respectively. The mouse AQP7 is 67% identical to the human AQP7. Such a lower conservation of AQP7 among species is unusual in the aquaporin family. The human AQP7 gene is composed of six exons distributing over 6.5 kb. The exon-intron boundaries are identical to those of the human AQP3 gene. The intron sizes are also similar. Moreover, chromosomal localization of AQP7 was assigned to 9p13 by fluorescent in situ hybridization, where AQP3 is also localized, suggesting that 9p13 may be another site of an aquaporin cluster.

摘要

从大鼠睾丸中分离出了第七种哺乳动物水通道蛋白（AQP7）的互补DNA（cDNA），并在晚期精子细胞的尾部证实了其表达（石桥等人，《生物化学杂志》272卷（1997年）第20782 - 20786页）。在此，我们报告小鼠和人类AQP7 cDNA以及人类AQP7基因的分离情况。人类AQP7基因与人类脂肪水通道蛋白（AQPap或AQP7L）相同。人类和小鼠AQP7推导的氨基酸序列分别与大鼠AQP7的氨基酸序列有68%和79%的同一性。小鼠AQP7与人类AQP7有67%的同一性。AQP7在物种间如此低的保守性在水通道蛋白家族中是不寻常的。人类AQP7基因由分布在6.5 kb上的六个外显子组成。外显子 - 内含子边界与人类AQP3基因的相同。内含子大小也相似。此外，通过荧光原位杂交将AQP7的染色体定位到9p13，AQP3也定位于此，这表明9p13可能是水通道蛋白簇的另一个位点。