小鼠胆碱激酶和胆碱/乙醇胺激酶的分子克隆：它们与各自大鼠同源物的序列比较。

Molecular cloning of mouse choline kinase and choline/ethanolamine kinase: their sequence comparison to the respective rat homologs.

作者信息

Aoyama C, Nakashima K, Ishidate K

机构信息

Department of Molecular Cell Biology, Medical Research Institute, Tokyo Medical and Dental University, 2-3-10 Kanda-surugadai, Chiyodaku, Tokyo 101-0062, Japan.

出版信息

Biochim Biophys Acta. 1998 Jul 31;1393(1):179-85. doi: 10.1016/s0005-2760(98)00062-9.

DOI:10.1016/s0005-2760(98)00062-9

PMID:9714798

Abstract

Complementary DNAs homologous to a rat 42-kDa choline/ethanolamine kinase [C. Aoyama et al., Biochim. Biophys. Acta 1390 (1998) 1-7] and to a 50-kDa choline kinase [T. Uchida and S. Yamashita, J. Biol. Chem. 267 (1992) 10156-10162] were isolated from a 17-day post coitum mouse embryo cDNA library and their sequences were compared with the two murine species, respectively. The nucleotide sequence homology (within the coding sequence) between mouse and rat 50-kDa choline kinases (96.0%) was considerably higher than that between their 42-kDa choline/ethanolamine kinases (92.4%). Northern blot and RT-PCR studies on several rat tissues demonstrated that both isozymes are expressed ubiquitously with the highest level in testis.

摘要

从妊娠17天的小鼠胚胎cDNA文库中分离出与大鼠42 kDa胆碱/乙醇胺激酶[C. Aoyama等人，《生物化学与生物物理学报》1390 (1998) 1 - 7]和50 kDa胆碱激酶[T. Uchida和S. Yamashita，《生物化学杂志》267 (1992) 10156 - 10162]同源的互补DNA，并分别将它们的序列与两种小鼠物种的序列进行比较。小鼠和大鼠50 kDa胆碱激酶之间（编码序列内）的核苷酸序列同源性（96.0%）明显高于它们42 kDa胆碱/乙醇胺激酶之间的同源性（92.4%）。对几种大鼠组织进行的Northern印迹和RT-PCR研究表明，两种同工酶在全身均有表达，在睾丸中的表达水平最高。