Site-directed mutagenesis using PCR-mediated introduction of silent mutations. - Suppr | 超能文献

Site-directed mutagenesis using PCR-mediated introduction of silent mutations.

作者信息

Kohli R M

机构信息

University of Michigan, Ann Arbor, USA.

出版信息

Biotechniques. 1998 Aug;25(2):184-8. doi: 10.2144/98252bm01.

DOI:10.2144/98252bm01

Abstract

摘要

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。

相似文献

1

Site-directed mutagenesis using PCR-mediated introduction of silent mutations.

Biotechniques. 1998 Aug;25(2):184-8. doi: 10.2144/98252bm01.

2

A novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis method.一种新型的基于PCR的、无需连接酶的、采用大引物法的定点诱变方法。

Anal Biochem. 2008 Apr 15;375(2):376-8. doi: 10.1016/j.ab.2007.12.013. Epub 2007 Dec 23.

3

Multiplex site-directed mutagenesis strategy including high-efficiency selection of the mutant PCR products.多重定点诱变策略，包括对突变型PCR产物的高效筛选。

Biotechnol Lett. 2007 Dec;29(12):1921-5. doi: 10.1007/s10529-007-9474-7. Epub 2007 Aug 9.

4

Seamless Ligation Cloning Extract (SLiCE) Method Using Cell Lysates from Laboratory Escherichia coli Strains and its Application to SLiP Site-Directed Mutagenesis.利用实验室大肠杆菌菌株细胞裂解物的无缝连接克隆提取物（SLiCE）方法及其在SLiP定点诱变中的应用。

Methods Mol Biol. 2017;1498:349-357. doi: 10.1007/978-1-4939-6472-7_23.

5

Site-directed mutagenesis by the megaprimer PCR method: variations on a theme for simultaneous introduction of multiple mutations.

Anal Biochem. 2002 Jul 15;306(2):346-9. doi: 10.1006/abio.2002.5689.

6

Two-stage PCR protocol allowing introduction of multiple mutations, deletions and insertions using QuikChange Site-Directed Mutagenesis.采用定点突变技术引入多个突变、缺失和插入的两步PCR方案。

Biotechniques. 1999 Apr;26(4):680-2. doi: 10.2144/99264st03.

7

An improved PCR-based method for site directed mutagenesis using megaprimers.一种改进的基于聚合酶链式反应（PCR）的使用大引物进行定点诱变的方法。

Mol Cell Probes. 1998 Dec;12(6):345-8. doi: 10.1006/mcpr.1998.0187.

8

PCR-mediated site-directed mutagenesis.聚合酶链式反应介导的定点诱变

Cold Spring Harb Protoc. 2013 Aug 1;2013(8):738-42. doi: 10.1101/pdb.prot076505.

9

Rapid gene splicing and multi-sited mutagenesis by one-step overlap extension polymerase chain reaction.一步重叠延伸聚合酶链反应快速基因拼接和多位点诱变。

Anal Biochem. 2012 Oct 1;429(1):76-8. doi: 10.1016/j.ab.2012.06.027. Epub 2012 Jul 4.

10

Site-directed mutagenesis by double polymerase chain reaction.通过双重聚合酶链反应进行的定点诱变

Mol Biotechnol. 1995 Feb;3(1):1-7. doi: 10.1007/BF02821329.

引用本文的文献

1

[A method for introducing mutations into large vectors].一种将突变引入大型载体的方法

Zhongguo Fei Ai Za Zhi. 2014 Jul 20;17(7):563-8. doi: 10.3779/j.issn.1009-3419.2014.07.12.

2

The role of threonine 37 in flavin reactivity of the old yellow enzyme.苏氨酸37在老黄色酶黄素反应性中的作用。

Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):3556-61. doi: 10.1073/pnas.96.7.3556.