Carey Michael F, Peterson Craig L, Smale Stephen T
Cold Spring Harb Protoc. 2013 Aug 1;2013(8):738-42. doi: 10.1101/pdb.prot076505.
Unlike traditional site-directed mutagenesis, this protocol requires only a single PCR step using full plasmid amplification to generate point mutants. The method can introduce small mutations into promoter sites and is even better suited for introducing single or double mutations into proteins. It is elegant in its simplicity and can be applied quite easily in any laboratory using standard protein expression vectors and commercially available reagents.
与传统的定点诱变不同，该方案仅需使用全质粒扩增进行单个PCR步骤即可产生点突变。该方法可将小突变引入启动子位点，甚至更适合于在蛋白质中引入单突变或双突变。它简单而巧妙，使用标准蛋白质表达载体和市售试剂，可在任何实验室轻松应用。
