Galili Y, Ben-Abraham R, Weinbroum A, Marmur S, Iaina A, Volman Y, Peer G, Szold O, Soffer D, Klausner J, Rabau M, Kluger Y
Department of Surgery B-C, Tel-Aviv Sourasky Medical Center, Israel.
J Trauma. 1998 Aug;45(2):222-5; discussion 225-6. doi: 10.1097/00005373-199808000-00004.
To investigate the effect of methylene blue, an inhibitor of oxygen radicals, on lung injury caused by reperfusion of ischemic tissue.
Intestinal ischemia-reperfusion injury was induced in rats by clamping the superior mesenteric artery for 1 hour. Thereafter, the experimental group was administered 1% methylene blue intraperitoneally and the control group received saline. After 4 hours, pulmonary histopathologic features were assessed, and lung wet-weight to dry-weight ratios and tissue xanthine oxidase were determined.
The control group suffered from severe pulmonary parenchymal damage, compared with slight damage in the experimental group. The number of sequestered neutrophils was significantly higher in the control group (319 +/- 60 polymorphonuclear cells per 10 high-power fields) than in the methylene blue-treated group (91 +/- 8 polymorphonuclear cells per 10 high-power fields; p < 0.001). The wet-weight to dry-weight ratio was significantly increased in the saline-treated rats compared with the methylene blue-treated group (6.19 +/- 0.28 vs. 5.07 +/- 0.21; p < 0.001). Xanthine oxidase activity was similar in both groups.
Methylene blue attenuated lung injury after intestinal ischemia-reperfusion. Inhibition of oxygen free radicals may be the protective mechanism.
研究氧自由基抑制剂亚甲蓝对缺血组织再灌注所致肺损伤的影响。
通过夹闭大鼠肠系膜上动脉1小时诱导肠缺血-再灌注损伤。此后，给实验组腹腔注射1%亚甲蓝，对照组注射生理盐水。4小时后，评估肺组织病理学特征，并测定肺湿重与干重之比及组织黄嘌呤氧化酶。
与实验组的轻微损伤相比，对照组出现严重的肺实质损伤。对照组中隔离的中性粒细胞数量（每10个高倍视野319±60个多形核细胞）显著高于亚甲蓝治疗组（每10个高倍视野91±8个多形核细胞；p<0.001）。与亚甲蓝治疗组相比，生理盐水处理的大鼠湿重与干重之比显著增加（6.19±0.28对5.07±0.21；p<0.001）。两组黄嘌呤氧化酶活性相似。
亚甲蓝减轻了肠缺血-再灌注后的肺损伤。抑制氧自由基可能是其保护机制。
