小鼠肝脏和肺谷胱甘肽S-环氧化物转移酶：苯巴比妥和3-甲基胆蒽给药的影响。

Mouse liver and lung glutathione s-epoxide transferase: effects of phenobarbital and 3-methylcholanthrene administration.

作者信息

Mukhtar H, Bresnick E

出版信息

Chem Biol Interact. 1976 Sep;15(1):59-67. doi: 10.1016/0009-2797(76)90128-9.

Abstract

The effects of 3-methylcholanthrene (3MC) and phenobarbital (PB) administration on the levels of glutathione-S-epoxide transferase activity in supernatant preparations of liver and lung were studied in a number of different strains of mice, C57Bl/6, C3H, C3H-f, Balb/c-, A+ and DBA/2+. Three epoxide substrates, 3MC-11,12-oxide, styrene oxide (SO) and 3,3,3-trichloro-1,2-epoxypropane (TCPO), were employed in this investigation. PB administration (75 mg/kg body weight for 3 days) resulted in 13-57% increases in enzyme activity in the liver supernatant but was ineffective in inducing activity in lung. 3MC administration (40 mg/kg body weight for 2 days) on the other hand was without any effect on glutathione-S-epoxide transferase activity in both liver and lung.

摘要

在多种不同品系的小鼠（C57Bl/6、C3H、C3H-f、Balb/c-、A+和DBA/2+）中，研究了给予3-甲基胆蒽（3MC）和苯巴比妥（PB）对肝脏和肺脏上清液制剂中谷胱甘肽-S-环氧化物转移酶活性水平的影响。本研究采用了三种环氧化物底物，即3MC-11,12-氧化物、氧化苯乙烯（SO）和3,3,3-三氯-1,2-环氧丙烷（TCPO）。给予PB（75毫克/千克体重，持续3天）使肝脏上清液中的酶活性增加了13%-57%，但对诱导肺脏中的活性无效。另一方面，给予3MC（40毫克/千克体重，持续2天）对肝脏和肺脏中的谷胱甘肽-S-环氧化物转移酶活性均无任何影响。