Valen G
Department of Thoracic Surgery, Karolinska Hospital, Stockholm, Sweden.
Acta Physiol Scand. 1998 Jul;163(3):219-25. doi: 10.1046/j.1365-201x.1998.00363.x.
Ischaemic preconditioning reduces infarct size, but the effects on cardiac function after global ischaemia are more controversial. Additionally, species differences may exist. The present study investigates the effects of preconditioning on cardiac performance in the globally ischaemic, Langendorff-perfused guinea-pig heart. Hearts were stabilized for 25 min, and divided into the following groups: (1) (n = 8) control perfusion for 16 min before 30-min global ischaemia and 30-min reperfusion, (2) (n = 7) two episodes of 3-min ischaemia and 5-min reperfusion before global ischaemia, (3) (n = 7) 5-min ischaemia and 10-min reperfusion before ischaemia, (4) (n = 8) control perfusion before 40-min ischaemia and 30-min reperfusion, (5) (n = 8) Preconditioning as group 2 before ischaemia as group 4, (6) (n = 9) Control perfusion before 50-min ischaemia and 30-min reperfusion, (7) (n = 10) Preconditioning as group 2 before ischaemia as group 6. A dose-dependent reduction of left ventricular systolic pressure, and increase of end-diastolic pressure was observed during reperfusion after 30-, 40- and 50-min ischaemia. Preconditioning did not influence these changes, nor did it attenuate the incidence of severe reperfusion arrhythmias or reduction of coronary flow. In conclusion, ischaemic preconditioning does not improve cardiac function during reperfusion of the globally ischaemic, isolated guinea-pig heart.
缺血预处理可减小梗死面积，但对全心缺血后心脏功能的影响更具争议性。此外，可能存在物种差异。本研究调查了预处理对全心缺血、Langendorff灌注豚鼠心脏心脏功能的影响。心脏稳定灌注25分钟后，分为以下几组：(1)（n = 8）在30分钟全心缺血和30分钟再灌注前进行16分钟的对照灌注；(2)（n = 7）在全心缺血前进行两次3分钟缺血和5分钟再灌注；(3)（n = 7）在缺血前进行5分钟缺血和10分钟再灌注；(4)（n = 8）在40分钟缺血和30分钟再灌注前进行对照灌注；(5)（n = 8）在第4组缺血前进行第2组的预处理；(6)（n = 9）在50分钟缺血和30分钟再灌注前进行对照灌注；(7)（n = 10）在第6组缺血前进行第2组的预处理。在30分钟、40分钟和50分钟缺血后的再灌注期间，观察到左心室收缩压呈剂量依赖性降低，舒张末期压力升高。预处理并未影响这些变化，也未减轻严重再灌注心律失常的发生率或冠状动脉血流的减少。总之，缺血预处理并不能改善全心缺血的离体豚鼠心脏再灌注期间的心脏功能。
