巴塞罗那脐带血库的胎盘血项目。

The placental blood program of the Barcelona Cord Blood Bank.

作者信息

Querol S, Gabarró M, Amat L, Gonzalez S, Gomez M D, de la Calle O, Madoz P, Badell I, Garcia J

机构信息

Cryobiology and Cell Therapy Department, Institut de Recerca Oncològica, Barcelona, Spain.

出版信息

Bone Marrow Transplant. 1998 Jul;22 Suppl 1:S3-5.

PMID:9715868

Abstract

Cord blood has recently become an alternative to bone marrow transplantation, generating the need for cord blood banks where large numbers of frozen cord blood units can be stored. The Barcelona Cord Blood Bank (bcB) was created in October 1995. Initially, several methods for volume reduction were tested, including Ficoll, Percoll, Gelatin and HES sedimentation. Of these, HES sedimentation (88% +/- 11 CD34+ cells recovery) was the one chosen for routine banking. Up to November 1997, the bank has processed 754 units with a median of 1.05 x 10(9) nucleated cells and 2.5 x 10(6) CD34+ cells stored per unit. Nine of these units have been shipped for transplantation.

摘要

脐带血最近已成为骨髓移植的替代物，这就产生了对脐带血库的需求，在脐带血库中可以储存大量冷冻的脐带血单位。巴塞罗那脐带血库（bcB）于1995年10月创建。最初，测试了几种体积减小方法，包括Ficoll、Percoll、明胶和羟乙基淀粉沉降法。其中，羟乙基淀粉沉降法（CD34 +细胞回收率为88%±11%）被选为常规建库方法。截至1997年11月，该库已处理了754个单位，每个单位储存的有核细胞中位数为1.05×10⁹，CD34 +细胞为2.5×10⁶。其中9个单位已被运送用于移植。