Drénou B, Choqueux C, El Ghalbzouri A, Blancheteau V, Toubert A, Charron D, Mooney N
INSERM U396, Institut des Cordeliers, Paris, France.
Bone Marrow Transplant. 1998 Jul;22 Suppl 1:S44-7.
The increasing use of umbilical cord blood in transplantation has led to a renewed interest in the immunological characterisation of this material. This study addresses the question of whether the CD95 molecule and its ligand are expressed and are functional in mediating cell death in cord blood mononuclear cells. These molecules have a crucial role in the homeostasis of haematopoietic cell populations in the adult and also contribute to graft-versus-host disease. CD95 is the most well studied receptor mediating a signal for cell death by apoptosis and its inducible ligand has been demonstrated to mediate cell death of multiple types of CD95 expressing cells. The object of this study was to examine whether cord blood mononuclear cells could behave either as targets for CD95-mediated cell death or as mediators of cell death due to the expression of CD95L. The results of this study lead us to suggest that cord blood mononuclear cells enjoy some immunological privilege due to the relatively low level of expression of CD95 (in comparison with adult peripheral blood lymphocytes) and the expression of the CD95 ligand.
脐带血在移植中的使用日益增加，这使得人们对该材料的免疫学特性重新产生了兴趣。本研究探讨了CD95分子及其配体在脐带血单个核细胞中是否表达以及在介导细胞死亡方面是否具有功能这一问题。这些分子在成人造血细胞群体的稳态中起着关键作用，并且也与移植物抗宿主病有关。CD95是研究最为深入的介导凋亡性细胞死亡信号的受体，其诱导性配体已被证明可介导多种表达CD95的细胞的死亡。本研究的目的是检验脐带血单个核细胞是否既可以作为CD95介导的细胞死亡的靶标，也可以由于CD95L的表达而作为细胞死亡的介质。本研究结果使我们认为，由于CD95的表达水平相对较低（与成人外周血淋巴细胞相比）以及CD95配体的表达，脐带血单个核细胞享有一定的免疫特权。
