交联DNA双链体——核酸外切酶稳定性及与κ轻链增强子的核酸转录因子（NF-κB）的相互作用

Cross-linked DNA duplexes--exonuclease stability and interaction with the nucleic transcription factor of the kappa light-chain enhancer (NF-kappaB).

作者信息

Antsypovich S, Kubareva E, Tashlitsky V, Oretskaya T, Shabarova Z

机构信息

Chemistry Department, M.V. Lomonosov Moscow State University, Russia.

出版信息

Eur J Biochem. 1998 Jul 15;255(2):414-21. doi: 10.1046/j.1432-1327.1998.2550414.x.

DOI:10.1046/j.1432-1327.1998.2550414.x

PMID:9716383

Abstract

Investigation of substrate properties of DNA duplexes with covalently connected strands has been performed. The kinetic measurements of the hydrolysis processes of these compounds by snake venom phosphodiesterase has been carried out. It has been shown that the exonuclease stability of cross-linked DNA duplexes is greatly increased as compared with analogs without linkages between strands. The interaction of cross-linked DNA duplexes with the p50 subunit of human nucleic transcription factor of the kappa light-chain enhancer (NF-kappaB) has been studied. The presence of linkages between strands did not prevent the binding of protein with DNA duplexes and, in specific cases, even promoted this process. The speculation of using cross-linked DNA duplexes as efficient decoys for DNA-binding proteins such as NF-kappaB from the point of view of sense technology are discussed.

摘要

已对具有共价连接链的DNA双链体的底物特性进行了研究。通过蛇毒磷酸二酯酶对这些化合物的水解过程进行了动力学测量。结果表明，与链间无连接的类似物相比，交联DNA双链体的核酸外切酶稳定性大大提高。研究了交联DNA双链体与人κ轻链增强子（NF-κB）核酸转录因子p50亚基的相互作用。链间连接的存在并不妨碍蛋白质与DNA双链体的结合，在特定情况下甚至促进了这一过程。从传感技术的角度讨论了使用交联DNA双链体作为DNA结合蛋白（如NF-κB）的有效诱饵的推测。