Tanaka H, Vickart P, Bertrand J R, Rayner B, Morvan F, Imbach J L, Paulin D, Malvy C
Laboratoire de Biochimie-Enzymologie, INSERM U140, CNRS URA147, Institut Gustave Roussy, Villejuif, France.
Nucleic Acids Res. 1994 Aug 11;22(15):3069-74. doi: 10.1093/nar/22.15.3069.
The potential use of alpha-beta-anomeric duplex oligonucleotides to inhibit transcription factor activity by the decoy approach is investigated in this report. Indeed, several alpha-beta-anomeric heteroduplexes display a sequence-specific interaction with the p50 subunit of the transcription factor NF kappa B. Used in a decoy approach, these duplexes interact strongly enough with this transcription factor to modulate the expression of a reporter gene, under the control of NF kappa B. However, all the alpha-beta-anomeric heteroduplexes do not interact with the p50 subunit; the sequence of the chirally natural beta-anomeric strand may explain the different recognition properties of the protein. The analysis of the appropriate beta-anomeric sequences is consistent with a preferential interaction of the p50 subunit with one strand of double-stranded DNA.
本报告研究了α-β异头双链寡核苷酸通过诱饵法抑制转录因子活性的潜在用途。实际上，几种α-β异头杂合双链体与转录因子NF-κB的p50亚基表现出序列特异性相互作用。在诱饵法中使用时，这些双链体与该转录因子的相互作用足够强烈，能够在NF-κB的控制下调节报告基因的表达。然而，并非所有的α-β异头杂合双链体都与p50亚基相互作用；手性天然β异头链的序列可能解释了蛋白质不同的识别特性。对合适的β异头序列的分析与p50亚基与双链DNA的一条链优先相互作用一致。
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