掩蔽、去掩蔽和调控性多聚腺苷酸化在小鼠卵母细胞中一个休眠mRNA的翻译控制中协同作用。
Masking, unmasking, and regulated polyadenylation cooperate in the translational control of a dormant mRNA in mouse oocytes.
作者信息
Stutz A, Conne B, Huarte J, Gubler P, Völkel V, Flandin P, Vassalli J D
机构信息
Department of Morphology, University of Geneva Medical School, 1211 Geneva 4, Switzerland.
出版信息
Genes Dev. 1998 Aug 15;12(16):2535-48. doi: 10.1101/gad.12.16.2535.
Abstract
The mechanisms responsible for translational silencing of certain mRNAs in growing oocytes, and for their awakening during meiotic maturation, are not completely elucidated. We show that binding of a approximately 80-kD protein to a UA-rich element in the 3' UTR of tissue-type plasminogen activator mRNA, a mouse oocyte mRNA that is translated during meiotic maturation, silences the mRNA in primary oocytes. Translation can be triggered by injecting a competitor transcript that displaces this silencing factor, without elongation of a pre-existing short poly(A) tail, the presence of which is mandatory. During meiotic maturation, cytoplasmic polyadenylation is necessary to maintain a poly(A) tail, but the determining event for translational activation appears to be the modification or displacement of the silencing factor.
摘要
导致生长中的卵母细胞某些mRNA翻译沉默以及减数分裂成熟过程中这些mRNA“唤醒”的机制尚未完全阐明。我们发现，一种约80-kD的蛋白质与组织型纤溶酶原激活物mRNA 3'UTR中富含UA的元件结合，该mRNA是一种在减数分裂成熟过程中进行翻译的小鼠卵母细胞mRNA，会使初级卵母细胞中的mRNA沉默。通过注射能取代这种沉默因子的竞争转录本可触发翻译，而无需延长预先存在的短聚腺苷酸尾，聚腺苷酸尾的存在是必需的。在减数分裂成熟过程中，细胞质聚腺苷酸化对于维持聚腺苷酸尾是必要的，但翻译激活的决定性事件似乎是沉默因子的修饰或取代。
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本文引用的文献
1
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Dev Biol. 1998 Jan 1;193(1):63-76. doi: 10.1006/dbio.1997.8785.
2
The 36-kilodalton embryonic-type cytoplasmic polyadenylation element-binding protein in Xenopus laevis is ElrA, a member of the ELAV family of RNA-binding proteins.非洲爪蟾中36千道尔顿的胚胎型细胞质聚腺苷酸化元件结合蛋白是ElrA，它是RNA结合蛋白ELAV家族的成员。
Mol Cell Biol. 1997 Nov;17(11):6402-9. doi: 10.1128/MCB.17.11.6402.
3
Nanos and pumilio establish embryonic polarity in Drosophila by promoting posterior deadenylation of hunchback mRNA.Nanos和pumilio通过促进驼背蛋白（hunchback）信使核糖核酸（mRNA）的尾部去腺苷酸化，在果蝇中建立胚胎极性。
Development. 1997 Aug;124(15):3015-23. doi: 10.1242/dev.124.15.3015.
4
Acquisition of meiotic competence in growing mouse oocytes is controlled at both translational and posttranslational levels.生长中的小鼠卵母细胞减数分裂能力的获得在翻译水平和翻译后水平均受到调控。
Dev Biol. 1997 Jul 1;187(1):43-54. doi: 10.1006/dbio.1997.8599.
5
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6
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Cell. 1997 May 16;89(4):597-606. doi: 10.1016/s0092-8674(00)80241-x.
7
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8
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