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An alpha/beta-HSQC-alpha/beta experiment for spin-state selective editing of IS cross peaks.

作者信息

Andersson P, Annila A, Otting G

机构信息

Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, S-171 77, Sweden.

出版信息

J Magn Reson. 1998 Aug;133(2):364-7. doi: 10.1006/jmre.1998.1492.

DOI:10.1006/jmre.1998.1492
PMID:9716480
Abstract

A generalized version of the TROSY experiment allows the spin-state selective editing of the four multiplet components of 15N-1H cross peaks of amide groups in proteins into four different subspectra, with no penalty in sensitivity. An improvement by 2 in sensitivity results, if only two of the four multiplet components are selected. Use of the experiment for the measurement of 1JHN coupling constants is discussed. A water flip-back version of the experiment is demonstrated with a 45 kDa fragment of 15N/2H labeled Staphylococcus aureus gyrase B. Copyright 1998 Academic Press.

摘要

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