Hong Y, Roy R, Ambros V
Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, USA.
Development. 1998 Sep;125(18):3585-97. doi: 10.1242/dev.125.18.3585.
C. elegans cki-1 encodes a member of the CIP/KIP family of cyclin-dependent kinase inhibitors, and functions to link postembryonic developmental programs to cell cycle progression. The expression pattern of cki-1::GFP suggests that cki-1 is developmentally regulated in blast cells coincident with G1, and in differentiating cells. Ectopic expression of CKI-1 can prematurely arrest cells in G1, while reducing cki-1 activity by RNA-mediated interference (RNAi) causes extra larval cell divisions, suggesting a role for cki-1 in the developmental control of G1/S. cki-1 activity is required for the suspension of cell cycling that occurs in dauer larvae and starved L1 larvae in response to environmental signals. In vulva precursor cells (VPCs), a pathway of heterochronic genes acts via cki-1 to maintain VPCs in G1 during the L2 stage.
秀丽隐杆线虫cki-1编码细胞周期蛋白依赖性激酶抑制剂CIP/KIP家族的一个成员，其功能是将胚胎后发育程序与细胞周期进程联系起来。cki-1::GFP的表达模式表明，cki-1在与G1期一致的胚细胞以及分化细胞中受到发育调控。CKI-1的异位表达可使细胞在G1期过早停滞，而通过RNA介导的干扰（RNAi）降低cki-1活性则会导致幼虫出现额外的细胞分裂，这表明cki-1在G1/S的发育控制中发挥作用。cki-1活性是滞育幼虫和饥饿L1幼虫响应环境信号而发生的细胞周期暂停所必需的。在外阴前体细胞（VPCs）中，一条异时性基因通路通过cki-1起作用，在L2期将VPCs维持在G1期。
