LeBoeuf R C, Caldwell M, Guo Y, Metz C, Davitz M A, Olson L K, Deeg M A
Departments of Medicine and Nutritional Sciences, University of Washington, Box 353410, Seattle, Washington 98195-3410, USA.
Mamm Genome. 1998 Sep;9(9):710-4. doi: 10.1007/s003359900851.
Glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) is an 110-kDa monomeric protein found in the circulation that is capable of degrading the GPI anchor utilized by dozens of cell-surface proteins in the presence of detergent. This protein is relatively abundant (5-10 microgram/ml in human serum), yet its sites of synthesis, gene structure, and overall function are unclear. It is our purpose to use the mouse system to determine its putative roles in lipid transport, pathogen control, and diabetes. We have isolated murine full-length cDNA for GPI-PLD from a pancreatic alpha cell library. The deduced amino acid sequence shows 74% homology to bovine and human GPI-PLD. There is a single structural gene (Gpld1) mapping to mouse Chromosome (Chr) 13, and among nine tissues, liver showed the greatest abundance of GPI-PLD mRNA. Genetic differences in serum GPI-PLD activity were seen among four mouse strains, and no correlation was seen between GPI-PLD activity and circulating levels of high density lipoproteins in these mice. This is the first report of map position and genetic regulation for Gpld1. This information will enable us to further study the expression and function of GPI-PLD in normal and pathological conditions.
糖基磷脂酰肌醇特异性磷脂酶D（GPI-PLD）是一种在循环系统中发现的110 kDa单体蛋白，在去污剂存在的情况下，它能够降解数十种细胞表面蛋白所利用的GPI锚。这种蛋白相对丰富（人血清中为5-10微克/毫升），但其合成位点、基因结构和整体功能尚不清楚。我们的目的是利用小鼠系统来确定其在脂质转运、病原体控制和糖尿病中的假定作用。我们从胰腺α细胞文库中分离出了小鼠GPI-PLD的全长cDNA。推导的氨基酸序列与牛和人GPI-PLD显示出74%的同源性。有一个单一的结构基因（Gpld1）定位于小鼠第13号染色体（Chr），在九个组织中，肝脏显示出最高丰度的GPI-PLD mRNA。在四个小鼠品系中观察到血清GPI-PLD活性的遗传差异，并且在这些小鼠中未观察到GPI-PLD活性与高密度脂蛋白循环水平之间的相关性。这是关于Gpld1图谱位置和遗传调控的首次报道。这些信息将使我们能够进一步研究GPI-PLD在正常和病理条件下的表达和功能。
