Howarth F C, Boyett M R, White E
Department of Physiology, University of Leeds, Leeds, LS2 9NQ, UK.
Pflugers Arch. 1998 Oct;436(5):804-6. doi: 10.1007/s004240050706.
Contraction and intracellular calcium ([Ca2+]i) transients were recorded using a video edge detector and fluorescence spectrophotometry, respectively, in rat ventricular myocytes at 22-24 degreesC stimulated at a frequency of 1 Hz. Application of the F-actin disrupter cytochalasin-D (Cyt-D) caused a large reduction in the amplitude of contraction and a small increase in the [Ca2+]i transient. These responses began within a few seconds of application and were complete after 2 min of exposure. Phase-plane relationships of contraction and [Ca2+]i were consistent with cytochalasin-D causing a decrease in myofilament responsiveness to Ca2+.
在22 - 24摄氏度下，以1赫兹的频率刺激大鼠心室肌细胞，分别使用视频边缘检测器和荧光分光光度法记录收缩和细胞内钙（[Ca2+]i）瞬变。应用F - 肌动蛋白破坏剂细胞松弛素 - D（Cyt - D）导致收缩幅度大幅降低，[Ca2+]i瞬变略有增加。这些反应在应用后几秒钟内开始，暴露2分钟后完成。收缩和[Ca2+]i的相平面关系与细胞松弛素 - D导致肌丝对Ca2+的反应性降低一致。
