Feingold N, Feingold J, Mouton D, Bouthillier Y, Stiffel C, Biozzi G
Eur J Immunol. 1976 Jan;6(1):43-51. doi: 10.1002/eji.1830060110.
Selective breeding for the character "agglutinin production to heterologous erythrocytes" was performed on random-bred populations of albino mice. Two types of selection were carried out: Selection I: the first 6 generations were immunized with sheep erythrocytes (SE), then SE and pigeon erythrocytes (PE) were alternated at each generation to avoid the interference of maternal antibody. Selection II: all generations were immunized with SE 60-70 days after weaning in order to eliminate the maternal antibody. Both were selected for the character agglutinin titer 14 days after immunization using an optimal dose of erythrocytes. Selection I. The mean response to SE of the foundation population was 7.36 +/- 1.60. The selection limit was attained between the 15th and the 20th generation. The F20--F30 were considered as homozygous for the character investigated. The mean SE agglutinin titers were 9.15 (1/2820) in the high responder line and 2 (1/20) in the low responder line. This corresponds to 140-fold interline difference in agglutinin titers. SE agglutinins determined in F1 hybrids, F2 hybrids and backcrosses obtained from F23--F29 demonstrated incomplete dominance of high responsiveness (23-29%). The inbreeding coefficient produced by close colony breeding was 0.55 in high and 0.71 in low responders at the 20th generation when both lines were homozygous with respect to agglutinin synthesis. Selection II. The mean response of the foundation population to SE was 7.79 +/- 1.56. The selection limit was attained in F14--F17 generations that were considered homozygous for the character investigated. The mean SE agglutinin titer was 9.2 (1/2900) in high responders and 2.8 (1/35) in low responders, which is an 83-fold interline difference in agglutinin titers. The results of both selections indicate that the character agglutinin synthesis is subject to polygenic regulation. The heritability (h2) of this character was estimated to be between 0.18 and 0.36 (range between the extreme values of Selections I and II.
对白化小鼠随机繁殖群体进行了针对“对异种红细胞产生凝集素”这一性状的选择性育种。进行了两种类型的选择：选择I：前6代用绵羊红细胞（SE）免疫，然后每代交替使用SE和鸽红细胞（PE）以避免母源抗体的干扰。选择II：所有世代在断奶后60 - 70天用SE免疫以消除母源抗体。两者均在使用最佳剂量红细胞免疫14天后针对凝集素效价这一性状进行选择。选择I。基础群体对SE的平均反应为7.36±1.60。在第15代到第20代之间达到了选择极限。F20 - F30被认为在所研究的性状上是纯合的。高反应品系中SE凝集素的平均效价为9.15（1/2820），低反应品系中为2（1/20）。这相当于凝集素效价在品系间有140倍的差异。在从F23 - F29获得的F1杂种、F2杂种和回交后代中测定的SE凝集素显示高反应性不完全显性（23 - 29%）。在第20代，当两个品系在凝集素合成方面均为纯合时，近交群体繁殖产生的近交系数在高反应品系中为0.55，在低反应品系中为0.71。选择II。基础群体对SE的平均反应为7.79±1.56。在被认为在所研究性状上是纯合的F14 - F17代达到了选择极限。高反应品系中SE凝集素的平均效价为9.2（1/2900），低反应品系中为2.8（1/35），这是凝集素效价在品系间83倍的差异。两种选择的结果均表明凝集素合成性状受多基因调控。该性状的遗传力（h2）估计在0.18至0.36之间（在选择I和II的极值范围之间）。
