[Nature of the mutations that determine the ability of Bacillus subtilis A-50 to sporulate at high glucose concentrations in the medium].

The ability of Bacillus subtilis A-50 to sporulate in the medium containing high glucose concentrations is caused by at least two mutation types: pts mutations and cat (or tgl) mutations, both of them affecting differently the level of alkaline proteinase synthesis. The decrease of the level of enzyme activity in the case of pts mutation (gluR3 mutant) occurs at the expense of glucose transport disturbance. The mutation cat (tgl) (mutant gluR5) causes the increase in enzyme synthesis at the expense of catabolic resistance to glucose of genes controlling alkaline proteinase synthesis and the spore formation in Bac. subtilis A-50. cat5(gluR5) and pts3(gluR3) mutations are located on the chromosome of Bac. subtilis in the region metD and argC respectively. The over-synthesis of alkaline proteinase characteristic of Bac. subtilis A-50 is controlled by the polygenic system, as the level of alkaline proteinase synthesis in argA+ transformants makes up 25% of the level of activity of the original strain. The productivity of Bac. subtilis A-50 can be enhanced by introducing an additional cat mutation.