Dobrzhanaskaia E O, Erokhina L I, Bol'shakova T N
Genetika. 1978;14(7):1175-84.
The ability of Bacillus subtilis A-50 to sporulate in the medium containing high glucose concentrations is caused by at least two mutation types: pts mutations and cat (or tgl) mutations, both of them affecting differently the level of alkaline proteinase synthesis. The decrease of the level of enzyme activity in the case of pts mutation (gluR3 mutant) occurs at the expense of glucose transport disturbance. The mutation cat (tgl) (mutant gluR5) causes the increase in enzyme synthesis at the expense of catabolic resistance to glucose of genes controlling alkaline proteinase synthesis and the spore formation in Bac. subtilis A-50. cat5(gluR5) and pts3(gluR3) mutations are located on the chromosome of Bac. subtilis in the region metD and argC respectively. The over-synthesis of alkaline proteinase characteristic of Bac. subtilis A-50 is controlled by the polygenic system, as the level of alkaline proteinase synthesis in argA+ transformants makes up 25% of the level of activity of the original strain. The productivity of Bac. subtilis A-50 can be enhanced by introducing an additional cat mutation.
枯草芽孢杆菌A - 50在含有高浓度葡萄糖的培养基中形成芽孢的能力是由至少两种突变类型引起的：pts突变和cat（或tgl）突变，这两种突变对碱性蛋白酶合成水平的影响不同。在pts突变（gluR3突变体）的情况下，酶活性水平的降低是以葡萄糖转运紊乱为代价的。cat（tgl）突变（突变体gluR5）导致酶合成增加，这是以控制碱性蛋白酶合成和枯草芽孢杆菌A - 50中芽孢形成的基因对葡萄糖的分解代谢抗性为代价的。cat5（gluR5）和pts3（gluR3）突变分别位于枯草芽孢杆菌染色体的metD和argC区域。枯草芽孢杆菌A - 50特有的碱性蛋白酶的过量合成受多基因系统控制，因为argA +转化体中碱性蛋白酶的合成水平占原始菌株活性水平的25%。通过引入额外的cat突变可以提高枯草芽孢杆菌A - 50的生产力。
