Laursen J, Larsen H J, Mikkelsen T R, Hjorth J P
Department of Molecular and Structural Biology, Arhus University, Denmark.
Genet Res. 1998 Jun;71(3):187-91. doi: 10.1017/s0016672398003280.
There are two known alleles of the mouse parotid secretory protein (PSP) gene: Pspa and Pspb. Pspa is carried by DBA/2J mice and Pspb is carried by C57BL/6J mice. Eighty-eight mice derived from a F1(C57BL/6J x DBA/2J) to DBA/2J backcross were analysed for PSP mRNA expression in the sublingual glands. Expression was found in heterozygous mice only. This indicates that only Pspb is expressed in this tissue. Furthermore, it maps the allele-specific sublingual gland determinant within 3.4 cM of Psp. Previous analysis of Pspb identified an enhancer-like region in position -4.6 to -3.1 kb that was necessary for transgene expression in the sublingual glands. Here it is shown that the corresponding region in Pspa enhances transgene expression in the sublingual glands as efficiently. The implications for regulation of PSP mRNA expression in the sublingual glands are discussed.
已知小鼠腮腺分泌蛋白（PSP）基因有两个等位基因：Pspa和Pspb。DBA/2J小鼠携带Pspa，C57BL/6J小鼠携带Pspb。对88只从F1（C57BL/6J×DBA/2J）回交至DBA/2J的小鼠进行了舌下腺中PSP mRNA表达分析。仅在杂合小鼠中发现表达。这表明该组织中仅表达Pspb。此外，它将等位基因特异性舌下腺决定簇定位在Psp的3.4 cM范围内。先前对Pspb的分析确定了位于-4.6至-3.1 kb位置的一个增强子样区域，该区域对于转基因在舌下腺中的表达是必需的。此处表明，Pspa中的相应区域同样有效地增强了转基因在舌下腺中的表达。讨论了其对舌下腺中PSP mRNA表达调控的意义。
