Poonia A, Purohit V D
Department of Veterinary Microbiology, CCS Haryana Agricultural University, Hisar, India.
Indian J Exp Biol. 1998 Apr;36(4):411-3.
Experimental model for chlamydial abortion was planned using pregnant guinea pigs to study whether the isolate of Chlamydia psittaci from natural cases of sheep abortion is able to cause experimental abortion in pregnant guinea pigs or not. Follow up and clinical observations like thermal reaction and haematological changes exhibited biphaic febrile response and marked leucopenia in both intraperitoneal group as well as intravaginal group. The laboratory examinations revealed presence of chlamydial intracytoplasmic inclusions in the impression smears of aborted foetal tissues. C. psittaci was reisolated successfully from all the aborted foetal tissues. It was observed that rate and severity of abortion were more in the intraperitoneal group as compared to intravaginal group. On correlating the results of clinical and laboratory examinations it was observed that guinea pig can be used as a suitable model for chlamydial abortion in future studies like cell mediate immune response and to see protective role of chlamydial vaccine, if any.
计划使用怀孕的豚鼠建立鹦鹉热衣原体流产的实验模型，以研究从自然流产绵羊病例中分离出的鹦鹉热衣原体是否能够在怀孕的豚鼠中引起实验性流产。随访以及诸如体温反应和血液学变化等临床观察显示，腹腔注射组和经阴道接种组均出现双相发热反应和明显的白细胞减少。实验室检查显示，流产胎儿组织的印片中有衣原体胞质内包涵体。从所有流产胎儿组织中均成功重新分离出鹦鹉热衣原体。观察到，与经阴道接种组相比，腹腔注射组的流产率和严重程度更高。将临床和实验室检查结果进行关联后发现，豚鼠可作为未来细胞介导免疫反应等研究中衣原体流产的合适模型，并可用于观察衣原体疫苗（若有）的保护作用。
