不同毛霉属物种中双链RNA分子和病毒样颗粒的检测

Detection of double-stranded RNA molecules and virus-like particles in different Mucor species.

作者信息

Vágvölgyi C, Magyar K, Papp T, Vastag M, Ferenczy L, Hornok L, Fekete C

机构信息

Department of Microbiology, Attila József University, Szeged, Hungary.

出版信息

Antonie Van Leeuwenhoek. 1998 Feb;73(2):207-10. doi: 10.1023/a:1000515905099.

DOI:10.1023/a:1000515905099

PMID:9717579

Abstract

The presence of double-stranded RNA elements was examined in 123 strains representing 18 Mucor species. These genetic elements were found to be present in 6 strains: 1 M. aligarensis, 1 M. hiemalis, 2 M. corticolus, 1 M. mucedo and 1 M. ramannianus. Electrophoretic separation of the nucleic acids revealed 4 different RNA patterns, with 1 to 5 discrete dsRNA bands. The molecular weights corresponding to these bands were 1.42-4.15 x 10(6) D. Using electronmicroscopy, for the first time the presence of virus like particles in Mucor species has been revealed.

摘要

在代表18种毛霉属物种的123个菌株中检测了双链RNA元件的存在情况。发现这些遗传元件存在于6个菌株中：1株阿里加毛霉、1株冬生毛霉、2株皮层毛霉、1株总状毛霉和1株拉曼毛霉。核酸的电泳分离显示出4种不同的RNA模式，有1至5条离散的双链RNA条带。与这些条带相对应的分子量为1.42 - 4.15×10⁶ D。通过电子显微镜首次揭示了毛霉属物种中存在病毒样颗粒。