Morrow J D, Scruggs J, Chen Y, Zackert W E, Roberts L J
Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232-6602, USA.
J Lipid Res. 1998 Aug;39(8):1589-93.
D2/E2-isoprostanes are prostaglandin D2/E2-like compounds that are produced in vivo as non-enzymatic products of free radical catalyzed peroxidation of arachidonic acid. One E2-isoprostane that should be formed is 15-E2t-isoprostane (8-iso-prostaglandin E2). 15-E2t-isoprostane has been shown to exert potent biological activity but proof that it is formed in vivo is lacking. Evidence is now presented that 15-E2t-isoprostane is formed in vivo by demonstrating that an endogenous E2-isoprostane with a retention time on capillary GC identical with that of 15-E2t-isoprostane co-chromatographs through four high resolving HPLC purification procedures with authentic radiolabeled 15-E2t-isoprostane.
D2/E2-异前列腺素是前列腺素D2/E2样化合物，作为花生四烯酸自由基催化过氧化的非酶促产物在体内产生。一种应该形成的E2-异前列腺素是15-E2t-异前列腺素（8-异前列腺素E2）。15-E2t-异前列腺素已被证明具有强大的生物活性，但缺乏其在体内形成的证据。现在有证据表明15-E2t-异前列腺素在体内形成，这是通过证明一种内源性E2-异前列腺素在毛细管气相色谱上的保留时间与15-E2t-异前列腺素相同，并且通过四个高分辨率高效液相色谱纯化程序与真实的放射性标记15-E2t-异前列腺素共色谱来实现的。
