Hou J, Major E O
Laboratory of Molecular Medicine and Neuroscience, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
J Neurovirol. 1998 Aug;4(4):451-6. doi: 10.3109/13550289809114545.
The effectiveness of nucleoside analogs in blocking viral multiplication was evaluated using an immortalized human neuroglial cell line capable of sustaining a persistent JCV infection, SVG-JC. Results from in situ DNA hybridization and hemagglutination assays performed on drug treated cultures were used as a measure of viral DNA replication and multiplication, respectively. Of the three drugs tested, Ara-C (cytosine arabinoside), AZT (3'-azido-3'-deoxythymidine), and cidofovir (S)-1-[3-hydroxy-2-(phosphonylmethoxypropyl] cytosine), only Ara-C showed a significant effect in decreasing active JCV replication and multiplication. In vitro data, using different cell types and virus strains have shown that specific drugs can indeed modulate viral infection. However, such modulation has not previously been demonstrated in those cells of the CNS which are specifically targeted by JCV. The SVG-JC cells represent a unique system with which further studies can be conducted on the effects of drugs on brain derived cells that are susceptible to viral infection.
使用能够维持持续性JCV感染的永生化人类神经胶质细胞系SVG-JC评估核苷类似物在阻断病毒增殖方面的有效性。对药物处理的培养物进行原位DNA杂交和血凝试验的结果分别用作病毒DNA复制和增殖的指标。在测试的三种药物阿糖胞苷（Ara-C）、齐多夫定（AZT）和西多福韦（(S)-1-[3-羟基-2-(膦酰甲氧基丙基)]胞嘧啶）中，只有阿糖胞苷在降低活跃的JCV复制和增殖方面显示出显著效果。使用不同细胞类型和病毒株的体外数据表明，特定药物确实可以调节病毒感染。然而，这种调节此前尚未在JCV特异性靶向的中枢神经系统细胞中得到证实。SVG-JC细胞代表了一个独特的系统，利用该系统可以进一步研究药物对易受病毒感染的脑源性细胞的影响。
