A simple method for site-directed mutagenesis with double-stranded plasmid DNA.

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超能文献

作者信息

Lai D, Zhu X, Pestka S

机构信息

Center for Neural Virology and Neural Oncology, Allegheny University of the Health Sciences, Philadelphia, PA, USA.

出版信息

Mol Biotechnol. 1998 Jun;9(3):235-41. doi: 10.1007/BF02915796.

DOI:10.1007/BF02915796

PMID:9718583

Abstract

摘要

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本文引用的文献

A simple and efficient method for site-directed mutagenesis with double-stranded plasmid DNA.一种用于双链质粒DNA定点诱变的简单高效方法。

Nucleic Acids Res. 1993 Aug 25;21(17):3977-80. doi: 10.1093/nar/21.17.3977.

Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA.使用M13衍生载体的寡核苷酸定向诱变：在任何DNA片段中产生点突变的高效通用方法。

Nucleic Acids Res. 1982 Oct 25;10(20):6487-500. doi: 10.1093/nar/10.20.6487.

Different base/base mismatches are corrected with different efficiencies by the methyl-directed DNA mismatch-repair system of E. coli.大肠杆菌的甲基化导向DNA错配修复系统对不同的碱基/碱基错配进行校正的效率不同。

Cell. 1984 Oct;38(3):879-87. doi: 10.1016/0092-8674(84)90283-6.

A simple and efficient procedure for saturation mutagenesis using mixed oligodeoxynucleotides.一种使用混合寡脱氧核苷酸进行饱和诱变的简单高效方法。

Gene. 1986;46(2-3):145-52. doi: 10.1016/0378-1119(86)90398-7.

Procedures for in vitro DNA mutagenesis of human leukocyte interferon sequences.

Methods Enzymol. 1986;119:403-15. doi: 10.1016/0076-6879(86)19060-4.

A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions.DNA片段的体外制备及特异性诱变的通用方法：蛋白质与DNA相互作用的研究

Nucleic Acids Res. 1988 Aug 11;16(15):7351-67. doi: 10.1093/nar/16.15.7351.

DNA mismatch-repair in Escherichia coli counteracting the hydrolytic deamination of 5-methyl-cytosine residues.大肠杆菌中的DNA错配修复可对抗5-甲基胞嘧啶残基的水解脱氨作用。

EMBO J. 1987 Jun;6(6):1809-15. doi: 10.1002/j.1460-2075.1987.tb02435.x.

Construction of mutant and chimeric genes using the polymerase chain reaction.利用聚合酶链反应构建突变基因和嵌合基因。

Nucleic Acids Res. 1989 Jan 25;17(2):723-33. doi: 10.1093/nar/17.2.723.

Creation of phosphorylation sites in proteins: construction of a phosphorylatable human interferon alpha.

Proc Natl Acad Sci U S A. 1989 Jan;86(2):558-62. doi: 10.1073/pnas.86.2.558.

Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS).DNA中任何点突变的分析。扩增阻滞突变系统（ARMS）。

Nucleic Acids Res. 1989 Apr 11;17(7):2503-16. doi: 10.1093/nar/17.7.2503.

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