Jaussaud P, Durix A, Videmann B, Vigié A, Bony S
INRA-ENVL Research Laboratory of Comparative Metabolism and Toxicology of Xenobiotics, National School of Veterinary Medicine of Lyon, Marcy l'Etoile, France.
J Chromatogr A. 1998 Jul 31;815(1):147-53. doi: 10.1016/s0021-9673(98)00002-8.
A rapid high-performance liquid chromatographic method for the determination of the mycotoxin ergovaline in ovine plasma is described here. Ergotamine was used as an internal standard. A simple extraction procedure with diethyloxide was carried out, before chromatography on a C8 column, with the excitation and emission wavelengths fixed at 250 and 420 nm respectively, on a fluorimetric detector. The method, which was found to be linear between 3.5 and 15 ng/ml, had good specificity, precision and accuracy. The limit of quantification and the limit of detection were 3.5 and 1.2 ng/ml, respectively. A preliminary application of the described assay to a plasma kinetic study, after intravenous administration of a single dose of ergovaline (17 micrograms/kg body mass) to four sheep, showed a very rapid decrease of the plasma ergovaline levels. The terminal half-life and the total clearance of the mycotoxin were found to be 23.6 min and 0.020 l/min kg-1 body mass, respectively.
本文介绍了一种用于测定绵羊血浆中霉菌毒素麦角缬氨酸的快速高效液相色谱法。以麦角胺作为内标。在C8柱上进行色谱分析之前，先用二乙氧基乙烷进行简单萃取，荧光检测器的激发波长和发射波长分别固定为250 nm和420 nm。该方法在3.5至15 ng/ml之间呈线性，具有良好的特异性、精密度和准确度。定量限和检测限分别为3.5 ng/ml和1.2 ng/ml。在对四只绵羊静脉注射单剂量麦角缬氨酸（17微克/千克体重）后，将所述测定法初步应用于血浆动力学研究，结果显示血浆麦角缬氨酸水平迅速下降。发现该霉菌毒素的末端半衰期和总清除率分别为23.6分钟和0.020升/分钟·千克体重。
