Xu D K, Zhou J H, Yuan Y S, Liu X Q, Huang S K
Jinling Hospital, Nanjing, China.
J Chromatogr. 1991 Jul 5;567(2):451-8. doi: 10.1016/0378-4347(91)80151-2.
A sensitive and simplified high-performance liquid chromatographic procedure has been developed for quantification of amiloride in rabbit plasma, as well as human plasma and urine. Following protein precipitation with perchloric acid, the supernatant was directly injected into a C18 Nucleosil column. The mobile phase consisted of methanol-water (45:55) containing 0.1 M perchloric acid, and the compound was quantitated using a fluorescence detector at excitation and emission wavelengths of 286 and 418 nm, respectively. The average recovery was 97.6%. The calibration curve was linear over the range 2.0-20.0 ng/ml. The limit of detection was 0.5 ng/ml.
已开发出一种灵敏且简化的高效液相色谱法，用于定量测定兔血浆、人血浆及尿液中的阿米洛利。用高氯酸进行蛋白沉淀后，将上清液直接注入C18核硅柱。流动相由含0.1 M高氯酸的甲醇-水（45:55）组成，该化合物分别在激发波长286 nm和发射波长418 nm下使用荧光检测器进行定量。平均回收率为97.6%。校准曲线在2.0 - 20.0 ng/ml范围内呈线性。检测限为0.5 ng/ml。
