Tietjen D N, Uramoto G Y, Tindall D J, Husmann D A
Department of Urology, Mayo Clinic, Rochester, Minnesota 55905, USA.
J Urol. 1998 Sep;160(3 Pt 2):1054-7; discussion 1079.
We determine whether testosterone therapy alters penile androgen receptor expression within the hypogonadotropic hypogonadal (HPG) micropenis.
In protocol 1 a strain of mice with micropenis due to congenital HPG and wild type litter mates were divided into testosterone treated and untreated groups. Treatment was initiated on day 15 of life. On day 90 of life (day 75 of treatment) the mice were sacrificed, and the penises were removed and weighed. In protocol 2 microphallic mice treated with testosterone were sacrificed on days 0, 6, 30, 60 and 75 after the initiation of treatment. Untreated HPG and wild type litter mates served as controls. At autopsy the penis was removed and weighed, and androgen receptor content was determined by Western immunoblotting.
Testosterone treatment resulted in 6-fold up regulation in HPG penile androgen receptor, approximately 1.4-fold higher than in the normal wild type pubescent penis. Testosterone induced and wild type pubescent penile androgen receptor up regulation was maintained for approximately 75 and 60 days, respectively. Despite improved HPG penile androgen receptor expression penile growth did not become normal. Average total penile weight plus or minus standard deviation was 7.2+/-3.5 mg. in untreated HPG mice. Testosterone significantly improved average HPG penile weight to 23.5+/-1.8 mg. (p <0.001). However, the testosterone treated micropenis failed to reach average normal penile size (38.6+/-2.6 mg., p <0.001).
Testosterone increases the concentration and duration of penile androgen receptor expression within the HPG micropenis. Despite this improvement microphallic HPG penile growth does not become normal.
我们确定睾酮治疗是否会改变性腺功能减退性性腺功能减退（HPG）小阴茎内的阴茎雄激素受体表达。
在方案1中，将因先天性HPG导致小阴茎的小鼠品系及其野生型同窝仔鼠分为睾酮治疗组和未治疗组。在出生后第15天开始治疗。在出生后第90天（治疗第75天）处死小鼠，取出阴茎并称重。在方案2中，对接受睾酮治疗的小阴茎小鼠在治疗开始后的第0、6、30、60和75天处死。未治疗的HPG小鼠和野生型同窝仔鼠作为对照。尸检时取出阴茎并称重，通过蛋白质免疫印迹法测定雄激素受体含量。
睾酮治疗导致HPG阴茎雄激素受体上调6倍，比正常野生型青春期阴茎高约1.4倍。睾酮诱导的和野生型青春期阴茎雄激素受体上调分别维持约75天和60天。尽管HPG阴茎雄激素受体表达有所改善，但阴茎生长并未恢复正常。未治疗的HPG小鼠阴茎平均总重量加减标准差为7.2±3.5毫克。睾酮显著提高了HPG小鼠阴茎平均重量至23.5±1.8毫克（p<0.001）。然而，接受睾酮治疗的小阴茎未能达到正常阴茎平均大小（38.6±2.6毫克，p<0.001）。
睾酮增加了HPG小阴茎内阴茎雄激素受体表达的浓度和持续时间。尽管有这种改善，小阴茎HPG的阴茎生长并未恢复正常。
