Taketa K
Department of Public Health, Okayama University Medical School, Japan.
Electrophoresis. 1998 Jul;19(10):1774-9. doi: 10.1002/elps.1150191039.
Multimodal application of lectin affinity electrophoresis of alpha-fetoprotein (AFP) glycoforms is reviewed. Crossed affinity immunoelectrophoresis developed by Bøg-Hansen and others was extended to tandem-lectin affinity electrophoresis by tandem lining of two different lectin gels and to mixed-lectin affinity electrophoresis. By introducing an antibody-affinity blotting technique for detection of separated glycoforms of AFP, several two-dimensional combinations of lectin affinity electrophoresis became possible: two different lectins for the first and second dimension electrophoresis, lectin-gradient affinity electrophoresis, and electrophoretic separation of lectin isoforms in the first-dimension electrophoresis, followed by affinity electrophoresis against the separated lectin isoforms. Usefulness of the different modalities of lectin affinity electrophoresis for several analytical purposes has been described.
综述了甲胎蛋白（AFP）糖型的凝集素亲和电泳的多模态应用。Bøg-Hansen等人开发的交叉亲和免疫电泳通过两种不同凝集素凝胶的串联排列扩展到串联凝集素亲和电泳和混合凝集素亲和电泳。通过引入用于检测AFP分离糖型的抗体亲和印迹技术，凝集素亲和电泳的几种二维组合成为可能：用于第一维和第二维电泳的两种不同凝集素、凝集素梯度亲和电泳，以及在第一维电泳中对凝集素同工型进行电泳分离，然后针对分离的凝集素同工型进行亲和电泳。已经描述了凝集素亲和电泳的不同模式在几种分析目的中的实用性。
