Taketa K
Department of Public Health, Okayama University Medical School, Japan.
Electrophoresis. 1998 Nov;19(15):2595-602. doi: 10.1002/elps.1150191506.
Alpha-Fetoprotein (AFP) glycoforms, defined as AFP with different chemical structures of carbohydrate, were analyzed by affinity electrophoresis with several lectins of known specificities against complex-type oligosaccharides. Serum AFP samples from cord blood on full-term delivery and from patients with hepatocellular carcinoma and extrahepatic malignancies including gastrointestinal tumors and yolk sac tumors were used. Two-dimensional lectin affinity electrophoresis and also lectin affinity chromatography coupled with lectin affinity electrophoresis were employed. More than ten AFP glycoforms were identified or characterized using the above-mentioned AFP samples. Known specificities of the lectins against complex-type oligosaccharides were refined or their additional specificities were found in this study. Lectin appeared to have specificity against carbohydrates by recognizing not only specific residues but also the whole carbohydrate molecule containing the residues, resulting in differential affinities for the lectin.
甲胎蛋白（AFP）糖型是指具有不同碳水化合物化学结构的AFP，通过使用几种对复合型寡糖具有已知特异性的凝集素进行亲和电泳来分析。使用了足月分娩时脐带血的血清AFP样本以及患有肝细胞癌和包括胃肠道肿瘤及卵黄囊瘤在内的肝外恶性肿瘤患者的血清AFP样本。采用了二维凝集素亲和电泳以及凝集素亲和色谱与凝集素亲和电泳联用的方法。使用上述AFP样本鉴定或表征了十多种AFP糖型。在本研究中，凝集素对复合型寡糖的已知特异性得到了细化，或者发现了它们的其他特异性。凝集素似乎通过不仅识别特定残基，而且识别包含这些残基的整个碳水化合物分子而对碳水化合物具有特异性，从而导致对凝集素的亲和力不同。
