空肠弯曲菌81116的脂多糖生物合成基因座
The lipopolysaccharide biosynthesis locus of Campylobacter jejuni 81116.
作者信息
Fry Ben N, Korolik Victoria, Ten Brinke Janna A, Pennings Maarten T T, Zalm Robert, Teunis Bart J J, Coloe Peter J, van der Zeijst Bernard A M
机构信息
Department of Bacteriology, Institute of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht UniversityYalelaan 1, 3584 CL UtrechtThe Netherlands.
Department of Applied BiologyRMIT GPO Box 2476V, Melbourne 3001Australia.
出版信息
Microbiology (Reading). 1998 Aug;144 ( Pt 8):2049-2061. doi: 10.1099/00221287-144-8-2049.
Most Campylobacter jejuni strains express lipo-oligosaccharides. Some strains also express lipopolysaccharides (LPS), with O-antigen-like carbohydrate repeats. C. jejuni 81116 expresses an LPS containing both lipo-oligosaccharides and O-antigen-like repeats, but nothing is known about the structure or sugar composition of these LPS species. A cosmid library of the genome of C. jejuni 81116 was constructed and probed with Campylobacter hyoilei genes involved in LPS synthesis. Five cosmids hybridized with the probe and two of these expressed C. jejuni 81116 LPS in Escherichia coli. By subcloning, a 16 kb DNA region was identified which contains the genetic information required to express C. jejuni LPS. DNA sequence analysis revealed 11 ORFs homologous to genes involved in LPS synthesis of other bacteria. They consisted of three homologues of sugar biosynthesis genes, two homologues of transport genes and six homologues of sugar transferases.
大多数空肠弯曲菌菌株表达脂寡糖。一些菌株还表达脂多糖（LPS），具有类似O抗原的碳水化合物重复序列。空肠弯曲菌81116表达一种同时含有脂寡糖和类似O抗原重复序列的LPS，但对这些LPS种类的结构或糖组成一无所知。构建了空肠弯曲菌81116基因组的黏粒文库，并用与LPS合成相关的猪肠弯曲菌基因进行探测。五个黏粒与探针杂交，其中两个在大肠杆菌中表达空肠弯曲菌81116 LPS。通过亚克隆，鉴定出一个16 kb的DNA区域，其包含表达空肠弯曲菌LPS所需的遗传信息。DNA序列分析揭示了11个与其他细菌LPS合成相关基因同源的开放阅读框（ORF）。它们由三个糖生物合成基因的同源物、两个转运基因的同源物和六个糖转移酶的同源物组成。
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