Ota H, Nakayama J, Momose M, Kurihara M, Ishihara K, Hotta K, Katsuyama T
Department of Laboratory Medicine, Shinshu University School of Medicine, Matsumoto, Nagano, Japan.
Histochem Cell Biol. 1998 Aug;110(2):113-9. doi: 10.1007/s004180050272.
The immunohistochemical reactivity of monoclonal antibodies raised against rat and pig gastric mucins (HIK1083, PGM36, and PGM37) was investigated in normal gastrointestinal tracts obtained from fish, amphibians, reptiles, birds, and mammals (including humans). These monoclonal antibodies exhibited highly selective reactivity with class III mucins, as identified by paradoxical concanavalin A stain, in the gastrointestinal tract of vertebrates. All three monoclonal antibodies reacted with the mucous neck cells and pyloric gland cells of amphibians, reptiles and mammals, the cardiac glands of reptiles and mammals, and Brunner's glands of mammls. The deep crypt secretory cells of the rat colon and certain goblet-type cells deep in crypts in the pig colon differed from the above pattern only in that they did not show immunoreactivity with monoclonal antibody PGM36. These data suggest that the development of class III mucin is a fundamental evolutionary characteristic of vertebrate gastric mucins. These monoclonal antibodies should prove useful for the investigation of cell differentiation among gastrointestinal mucous cells and for the biochemical analysis of gastrointestinal mucins in different species.
研究了针对大鼠和猪胃粘蛋白产生的单克隆抗体（HIK1083、PGM36和PGM37）在从鱼类、两栖动物、爬行动物、鸟类和哺乳动物（包括人类）获取的正常胃肠道中的免疫组织化学反应性。这些单克隆抗体在脊椎动物胃肠道中对经伴刀豆球蛋白A反常染色鉴定的III类粘蛋白表现出高度选择性反应性。所有三种单克隆抗体均与两栖动物、爬行动物和哺乳动物的粘液颈细胞和幽门腺细胞、爬行动物和哺乳动物的贲门腺以及哺乳动物的 Brunner 腺发生反应。大鼠结肠的深部隐窝分泌细胞和猪结肠隐窝深处的某些杯状细胞型细胞与上述模式的不同之处仅在于它们不与单克隆抗体PGM36表现出免疫反应性。这些数据表明III类粘蛋白的发育是脊椎动物胃粘蛋白的一个基本进化特征。这些单克隆抗体应被证明对研究胃肠道粘液细胞之间的细胞分化以及对不同物种胃肠道粘蛋白的生化分析有用。
