Suganuma T, Katsuyama T, Tsukahara M, Tatematsu M, Sakakura Y, Murata F
Am J Anat. 1981 Jun;161(2):219-38. doi: 10.1002/aja.1001610206.
Combinations of recently developed paradoxical concanavalin-A staining (PCS) and other routine histochemical procedures have made it possible to classify mucosubstances more precisely. By taking advantage of these sequences, the present study was undertaken both to characterize the mucinous contents of alimentary tracts of several animal species, and to compare the variability of epithelial mucosubstances with special reference to the mucous neck cells of the stomach. The alimentary tracts obtained from 7 species of fish, 5 amphibians, 3 reptiles, 4 birds, and 6 mammals were used. Acidity of epithelial mucosubstances was highly variable even among the corresponding mucous cells. On the contrary, concanavalin-A reactivity seemed to be consistent with each cell and species. The mucous neck cells, which were observed in amphibians, reptiles, and mammals, consistently exhibited a characteristic mucosubstance with stable class-III reactivity by PCS. Inversely, stable class-III reactivity was found only in species possessing mucous neck cells, and was widely distributed in the esophageal glands of the frog, snake, and man; in esophageal mucous cells of the frog and skink; in pyloric glands of amphibia, reptiles, and mammals; and in Brunner's glands of mammals. These mucous cells seem to form an unique group with morphological and histochemical similarities. It is likely that the stable class-III reactivity by PCS is a hallmark of the mucous neck cells and related glands and that, in addition, concanavalin-A reactivity of mucosubstances is evolutionarily more fundamental characteristic.
最近开发的伴刀豆球蛋白A反常染色法（PCS）与其他常规组织化学方法的结合，使得更精确地对粘膜物质进行分类成为可能。利用这些方法，本研究旨在对几种动物消化道的粘液成分进行特征描述，并特别参照胃的粘液颈细胞，比较上皮粘膜物质的变异性。使用了从7种鱼类、5种两栖动物、3种爬行动物、4种鸟类和6种哺乳动物获取的消化道。即使在相应的粘液细胞中，上皮粘膜物质的酸度也高度可变。相反，伴刀豆球蛋白A反应性似乎在每个细胞和物种中都是一致的。在两栖动物、爬行动物和哺乳动物中观察到的粘液颈细胞，通过PCS始终表现出具有稳定的III类反应性的特征性粘膜物质。相反，仅在具有粘液颈细胞的物种中发现了稳定的III类反应性，并且广泛分布于青蛙、蛇和人类的食管腺中；青蛙和石龙子的食管粘液细胞中；两栖动物、爬行动物和哺乳动物的幽门腺中；以及哺乳动物的布伦纳腺中。这些粘液细胞似乎形成了一个具有形态学和组织化学相似性的独特群体。PCS的稳定III类反应性很可能是粘液颈细胞和相关腺体的标志，此外，粘膜物质的伴刀豆球蛋白A反应性在进化上是更基本的特征。
