Campbell L C, Raison J K, Brady C J
J Bioenerg Biomembr. 1976 Jun;8(3):121-9. doi: 10.1007/BF00748958.
The state-3 rate of respiration of potato tuber mitochondria is inhibited by concentrations of KCl or NaCl above 125 mM, and by concentrations of sucrose, lactose, or maltose above 500 mM, but not at all by mannitol, glucose, glycine, or proline up to a concentration of 1500 mM in the medium. Mitochondria from cauliflower, beetroot, cucumber, rock melon, and watermelon behave very similarly to those from potato tuber. The variable response to different solutes proves that the reduction in respiration is not a simple function of the chemical potential of water in the medium. Disruption of potato mitochondria by ultrasonic vibration does not relieve the inhibition of succinate oxidation caused by KCl or sucrose. However, treatment with detergent abolishes completely the inhibition of respiration by sucrose. Inhibition of succinate dehydrogenase [Succinate:PMS, oxidoreductase (EC.1.3.99.1)] and malate dehydrogenase [L-Malate:NAD oxidoreductase (EC.1.1.1.37)] activities by sucrose is less than the inhibition of succinate- and malate-dependent oxygen uptake by the potato mitochondria. Limited substrate uptake and, alternatively, reduced electron flow as a consequence of a direct effect of solute on the mitochondrial membrane are considered as possible mechanisms of inhibition.
当氯化钾或氯化钠浓度高于125 mM，以及蔗糖、乳糖或麦芽糖浓度高于500 mM时，马铃薯块茎线粒体的状态3呼吸速率会受到抑制，但在培养基中甘露醇、葡萄糖、甘氨酸或脯氨酸浓度高达1500 mM时，对其呼吸速率则毫无影响。来自花椰菜、甜菜根、黄瓜、甜瓜和西瓜的线粒体表现与马铃薯块茎线粒体非常相似。对不同溶质的反应各异，这表明呼吸作用的降低并非培养基中水的化学势的简单函数。超声振动破坏马铃薯线粒体并不能缓解由氯化钾或蔗糖引起的琥珀酸氧化抑制。然而，用去污剂处理可完全消除蔗糖对呼吸作用的抑制。蔗糖对琥珀酸脱氢酶[琥珀酸：吩嗪硫酸甲酯氧化还原酶（EC.1.3.99.1）]和苹果酸脱氢酶[L-苹果酸：NAD氧化还原酶（EC.1.1.1.37）]活性的抑制作用小于对马铃薯线粒体中琥珀酸和苹果酸依赖的氧气摄取的抑制作用。底物摄取受限，或者由于溶质对线粒体膜的直接作用导致电子流减少，被认为是可能的抑制机制。
