Departments of Crop Science and Botany and Crops Research Division, United States Department of Agriculture, North Carolina State University, Raleigh, North Carolina 27607.
Plant Physiol. 1971 Apr;47(4):532-6. doi: 10.1104/pp.47.4.532.
The properties of the adenosine triphosphatase activity associated with tightly coupled, time-stable mung bean (Phaseolus aureus Roxb.) mitochondria resemble those of intact animal mitochondria. Induction of adenosine triphosphatase activity by 2,4-dinitrophenol was inhibited by oligomycin, oxidizable substrates, and high concentrations of sucrose. Upon sonication, high rates of endogenous adenosine triphosphate hydrolysis resulted, an absolute requirement for Mg(2+) was manifested, stimulation by 2,4-dinitrophenol and inhibition by sucrose were eliminated, but sensitivity to oligomycin was retained.
与紧密偶联、时间稳定的绿豆(Phaseolus aureus Roxb.)线粒体相关的三磷酸腺苷酶活性的性质类似于完整动物线粒体的性质。2,4-二硝基苯酚诱导的三磷酸腺苷酶活性被寡霉素、可氧化的底物和高浓度的蔗糖抑制。超声处理后,会导致内源性三磷酸腺苷水解的高速率,表现出对 Mg(2+)的绝对需求,2,4-二硝基苯酚的刺激和蔗糖的抑制作用被消除,但对寡霉素的敏感性仍然保留。