Kataoka N, Tokiwa Y
Research and Development Department, Ebara Corporation, Kanagawa, Japan.
J Appl Microbiol. 1998 Mar;84(3):357-67. doi: 10.1046/j.1365-2672.1998.00349.x.
Of 10 strains of mannanase-producing anaerobic bacteria isolated from soils and methanogenic sludges, Clostridium tertium KT-5A, which was isolated from lotus soil, produced high amounts of extracellular beta-1,4-mannanase. The isolate was an aerotolerant anaerobe without quinon systems; the cell growth cultivated with no addition of reducing agents was also stable. High yields of mannanase were obtained by inducing enzyme production with galactomannan guar gum and beef extract/peptone as carbon and nitrogen sources, respectively. Fermentation end products on galactomannan fermentation were formate, acetate, lactate, butyrate, carbon dioxide and hydrogen. The extracellular mannanase displayed high activity on galactomannans of locust bean gum galactose/mannose (G/M) ratio 1:4 and spino gum (G/M 1:3), but weak activity on guar gum galactomannan (G/M 1:2) and konjac glucomannan. As far as is known, this is the first report on the isolation of an active mannanase-producing anaerobic bacterium from natural environments.
从土壤和产甲烷污泥中分离出的10株产甘露聚糖酶厌氧细菌中，从莲藕土壤中分离出的第三梭菌KT-5A能产生大量胞外β-1,4-甘露聚糖酶。该分离株是一种无醌系统的耐氧厌氧菌；在不添加还原剂的情况下培养，细胞生长也很稳定。分别以半乳甘露聚糖瓜尔胶和牛肉膏/蛋白胨作为碳源和氮源诱导产酶，可获得高产甘露聚糖酶。半乳甘露聚糖发酵的终产物为甲酸盐、乙酸盐、乳酸盐、丁酸盐、二氧化碳和氢气。胞外甘露聚糖酶对刺槐豆胶半乳甘露聚糖（半乳糖/甘露糖比例为1:4）和刺云实胶（半乳糖/甘露糖比例为1:3）表现出高活性，但对瓜尔胶半乳甘露聚糖（半乳糖/甘露糖比例为1:2）和魔芋葡甘露聚糖活性较弱。据所知，这是首次从自然环境中分离出具有活性的产甘露聚糖酶厌氧细菌的报道。
