Jiang Baohang, Sun Zhen, Hou Yingmin, Yang Lan, Yang Fan, Chen Xiaoyi, Li Xianzhen
a School of Biological Engineering , Dalian Polytechnic University , Dalian , China.
Prep Biochem Biotechnol. 2016 Jul 3;46(5):495-500. doi: 10.1080/10826068.2015.1084516.
Endo-β-mannanase, catalyzing the random hydrolysis of β-1,4-mannosidic linkage in the backbone of (hetero) mannan, can increase feed conversion efficiency of animal feed or form functional mannanooligosaccharides. In this study, a gram-positive, straight-rod, facultative anaeorobic bacterium producing endo-β-mannanase was isolated from soil sample. The isolate only fermented glucose, galactose, sorbose, and raffinose to acid. The test in hydrogen sulfide production was positive. Combining the data acquired from phenotypic analysis and phylogenetic analysis based on 16S rRNA gene sequences, this strain presumably represented a novel species of the genus Bacillus and was designated as LX114. The strain LX114 could break down guar gum molecules, leading to a rapid decrease of the viscosity of guar gum solutions. Endo-β-mannanase activity was also detected in the culture supernatant. The isolate LX114 would be useful for potential application in degrading plant cell walls for increasing feed conversion efficiency and formation of functional oligosaccharides.
内切β-甘露聚糖酶可催化（杂）甘露聚糖主链中β-1,4-甘露糖苷键的随机水解，能提高动物饲料的饲料转化效率或形成功能性甘露寡糖。在本研究中，从土壤样品中分离出一株产内切β-甘露聚糖酶的革兰氏阳性、直杆状、兼性厌氧菌。该分离株仅能将葡萄糖、半乳糖、山梨糖和棉子糖发酵产酸。硫化氢产生试验呈阳性。结合表型分析和基于16S rRNA基因序列的系统发育分析所获得的数据，该菌株可能代表芽孢杆菌属的一个新物种，并被命名为LX114。菌株LX114能够分解瓜尔胶分子，导致瓜尔胶水溶液的粘度迅速降低。在培养上清液中也检测到了内切β-甘露聚糖酶活性。分离株LX114在降解植物细胞壁以提高饲料转化效率和形成功能性寡糖方面具有潜在的应用价值。
