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狂犬病病毒在猪肾细胞单层上的蚀斑测定。

Plaque assay of rabies virus on porcine kidney cell monolayers.

作者信息

Strating A, Mitzel J R, Slater E A

出版信息

J Clin Microbiol. 1976 Aug;2(2):126-8.

PMID:972180
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC274146/
Abstract

The ERA strain of rabies virus was adapted to growth on monolayers of a porcine kidney cell line (PK-2A). A reproducible plaque assay system was subsequently developed, which appears to be satisfactory for conducting plaque neutralization tests.

摘要

狂犬病病毒的ERA毒株经适应后可在猪肾细胞系(PK-2A)的单层细胞上生长。随后开发了一种可重复的蚀斑测定系统,该系统似乎足以用于进行蚀斑中和试验。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f79/274146/ee464d3a4a49/jcm00229-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f79/274146/ee464d3a4a49/jcm00229-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f79/274146/ee464d3a4a49/jcm00229-0063-a.jpg

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本文引用的文献

1
Propagation of Rabies Virus in Pig Kidney Cell Culture.狂犬病病毒在猪肾细胞培养物中的增殖
Can Vet J. 1964 Apr;5(4):84-7.
2
Effect of polyions on the infectivity of rabies virus in tissue culture: construction of a single-cycle growth curve.聚离子对狂犬病病毒在组织培养中感染性的影响:单周期生长曲线的构建
J Virol. 1967 Feb;1(1):145-51. doi: 10.1128/JVI.1.1.145-151.1967.
3
Reproducible plaquing system for rabies, lymphocytic choriomeningitis,k and other ribonucleic acid viruses in BHK-21-13S agarose suspensions.用于狂犬病、淋巴细胞性脉络丛脑膜炎病毒及其他核糖核酸病毒在BHK - 21 - 13S琼脂糖悬液中的可重复性蚀斑形成系统。
J Virol. 1967 Dec;1(6):1224-6. doi: 10.1128/JVI.1.6.1224-1226.1967.
4
A rapid reproducible test for determining rabies neutralizing antibody.一种用于测定狂犬病中和抗体的快速可重复检测方法。
Bull World Health Organ. 1973 May;48(5):535-41.
5
Chronic rabies virus infection of cell cultures.细胞培养物的慢性狂犬病病毒感染
Infect Immun. 1972 Dec;6(6):988-95. doi: 10.1128/iai.6.6.988-995.1972.
6
Laboratory techniques in rabies: cell monolayer plaque test.狂犬病实验室技术:细胞单层蚀斑试验
Monogr Ser World Health Organ. 1973(23):339-42.
7
Plaque assay for rabies serogroup viruses in vero cells.在 vero 细胞中对狂犬病血清群病毒进行蚀斑测定。
J Clin Microbiol. 1975 Feb;1(2):241-2. doi: 10.1128/jcm.1.2.241-242.1975.