Buffenmyer C L, Rycheck R R, Yee R B
J Clin Microbiol. 1976 Sep;4(3):239-44. doi: 10.1128/jcm.4.3.239-244.1976.
High-titer preparations of Klebsiella bacteriocins (klebocins) were obtained by using mitomycin to induce standard strains of Klebsiella. Of 296 clinical isolates of Klebsiella, 67% could be typed on the basis of their sensitivity to klebocins. The method proposed in this paper affords a standard basis for the further development of klebocin typing as a suitable procedure for hospital laboratories concerned with epidemiological investigations of hospital-associated infections. Evidence is also provided to show that high-titered klebocin typing can be used in conjunction with biochemical typing to provide a sensitive epidemiological marker for Klebsiella.
通过使用丝裂霉素诱导克雷伯菌标准菌株,获得了高滴度的克雷伯菌细菌素(klebocins)制剂。在296株克雷伯菌临床分离株中,67%可根据其对klebocins的敏感性进行分型。本文提出的方法为进一步发展klebocin分型提供了标准依据,使其成为适合医院实验室进行医院感染流行病学调查的合适程序。同时也提供了证据表明,高滴度的klebocin分型可与生化分型结合使用,为克雷伯菌提供一个敏感的流行病学标志物。