肺炎克雷伯菌临床分离株的生化与血清学联合分型
Combined biochemical and serological typing of clinical isolates of Klebsiella.
作者信息
Rennie R P, Duncan I B
出版信息
Appl Microbiol. 1974 Oct;28(4):534-9. doi: 10.1128/am.28.4.534-539.1974.
Abstract
In a series of 640 strains of Klebsiella isolated from clinical specimens over a 7-month period, there were sufficient biochemical differences between strains to allow a biochemical typing system to be established. Biochemical tests were done in solid media inoculated with a modified Steers inocula replicator. Biotypes were designated by a numerical coding system; 29 distinct biotypes were found among the 640 strains of Klebsiella. Serotyping of 270 of the strains was done by the Quellung reaction, and 40 capsular types were identified. Numerical biotypes and serotypes of strains appeared to vary independently. When used in conjunction, the two methods subdivided the strains into many more distinct types than either used alone. With the combined method over 100 types of Klebsiella were distinguished among the 270 isolates.
摘要
在7个月期间从临床标本中分离出的640株克雷伯菌中，菌株之间存在足够的生化差异，从而能够建立一个生化分型系统。生化试验在接种了改良Steers接种物复制器的固体培养基中进行。生物型由数字编码系统指定；在640株克雷伯菌中发现了29种不同的生物型。通过荚膜肿胀反应对其中270株菌株进行了血清分型，鉴定出40种荚膜类型。菌株的数字生物型和血清型似乎独立变化。当联合使用时，这两种方法将菌株细分为比单独使用任何一种方法都多得多的不同类型。使用联合方法在270株分离株中区分出了100多种克雷伯菌类型。
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