Tarazi F I, Campbell A, Baldessarini R J
Mailman Research Center, McLean Division of Massachusetts General Hospital, Belmont 02178, USA.
Neuroscience. 1998 Nov;87(1):1-4. doi: 10.1016/s0306-4522(98)00220-6.
Changes in D1-, D2- and D4-like dopamine receptor binding in rat caudate-putamen, nucleus accumbens and hippocampus were examined by quantitative in vitro receptor autoradiography after unilateral injections of kainic acid into hippocampus to degenerate local intrinsic neurons and remove hippocampal-striatal projections. Radioligands were: [3H]SCH-23390 for D1-like (D1/D5) receptors, [3H]nemonapride alone for D2-like (D2/D3/D4) receptors and with 300 nM S[-]-raclopride and other masking agents for D4-like receptors. Kainic acid injections produced massive local hippocampal losses of D1-like (75%), D2-like (77%) and D4-like (79%) receptor binding but did not significantly alter D1-, D2- or D4-like receptor density in caudate-putamen or nucleus accumbens. Most D1-, D2- and D4-like receptors in rat hippocampus evidently arise on intrinsic postsynaptic neurons whereas none was detected on presynaptic terminals of hippocampal-striatal afferents innervating the striatal complex.
在将海藻酸单侧注射入海马体以使局部内在神经元退化并去除海马体 - 纹状体投射后，通过定量体外受体放射自显影术检测大鼠尾状核 - 壳核、伏隔核和海马体中D1 -、D2 - 和D4样多巴胺受体结合的变化。放射性配体分别为：用于D1样（D1 / D5）受体的[3H] SCH - 23390，单独用于D2样（D2 / D3 / D4）受体的[3H]奈莫必利，以及用于D4样受体的含300 nM S -（-）雷氯必利和其他掩蔽剂的溶液。注射海藻酸导致海马体局部D1样（75%）、D2样（77%）和D4样（79%）受体结合大量丧失，但未显著改变尾状核 - 壳核或伏隔核中D1 -、D2 - 或D4样受体密度。大鼠海马体中的大多数D1 -、D2 - 和D4样受体显然出现在内在突触后神经元上，而在支配纹状体复合体的海马体 - 纹状体传入神经的突触前终末未检测到这些受体。
