利用模板聚合实现DNA-聚合物复合物的自组装。
Self-assembly of DNA-polymer complexes using template polymerization.
作者信息
Trubetskoy V S, Budker V G, Hanson L J, Slattum P M, Wolff J A, Hagstrom J E
机构信息
Mirus Corporation, 545 Science Drive, Madison, WI 53711, USA and University of Wisconsin-Madison, Madison, WI 53705, USA.
出版信息
Nucleic Acids Res. 1998 Sep 15;26(18):4178-85. doi: 10.1093/nar/26.18.4178.
Abstract
The self-assembly of supramolecular complexes of nucleic acids and polymers is of relevance to several biological processes including viral and chromatin formation as well as gene therapy vector design. We now show that template polymerization facilitates condensation of DNA into particles that are <150 nm in diameter. Inclusion of a poly(ethylene glycol)-containing monomer prevents aggregation of these particles. The DNA within the particles remains biologically active and can express foreign genes in cells. The formation or breakage of covalent bonds has until now not been employed to compact DNA into artificial particles.
摘要
核酸与聚合物超分子复合物的自组装与多种生物过程相关，包括病毒和染色质形成以及基因治疗载体设计。我们现在表明，模板聚合有助于将DNA凝聚成直径小于150纳米的颗粒。包含含聚乙二醇的单体可防止这些颗粒聚集。颗粒内的DNA保持生物活性，并能在细胞中表达外源基因。迄今为止，尚未采用共价键的形成或断裂将DNA压缩成人工颗粒。
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