Dinning A J, al-Adham I S, Austin P, Collier P J
School of Molecular and Life Sciences, University of Abertay Dundee, Jordan.
Lett Appl Microbiol. 1998 Jul;27(1):1-4. doi: 10.1046/j.1472-765x.1998.00376.x.
Sodium pyrithione and zinc pyrithione (NaPT and ZnPT, respectively) are widely used as cosmetic preservatives and metal chelating agents. They are commonly assayed using thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). However, a simple quantitative colorimetric assay has not been previously reported for these compounds. This paper describes the development of a spectrophotometric assay for the quantification of the pyrithiones which is based on the chelation of copper (II) ions by the biocides. This assay was developed in order to facilitate the determination of the distribution of these biocides in the Gram-negative bacteria Escherichia coli NCIMB 10,000 and Pseudomonas aeruginosa NCIMB 10,548. Sodium pyrithione was exhibited only in the cytosol of E. coli and Ps. aeruginosa. Zinc pyrithione, however, was assayed in the cytosol of both bacteria and was found in the cell envelope of Ps. aeruginosa. These findings suggest that the pyrithione biocides are active within bacterial cells as well as at the cell membrane.
巯氧吡啶钠和巯氧吡啶锌（分别为NaPT和ZnPT）被广泛用作化妆品防腐剂和金属螯合剂。它们通常采用薄层色谱法（TLC）和高效液相色谱法（HPLC）进行测定。然而，此前尚未报道过针对这些化合物的简单定量比色法。本文描述了一种基于杀菌剂对铜（II）离子的螯合作用来定量测定巯氧吡啶类化合物的分光光度法。开发该测定方法是为了便于确定这些杀菌剂在革兰氏阴性菌大肠杆菌NCIMB 10,000和铜绿假单胞菌NCIMB 10,548中的分布情况。巯氧吡啶钠仅在大肠杆菌和铜绿假单胞菌的胞质溶胶中出现。然而，巯氧吡啶锌在两种细菌的胞质溶胶中均有检测到，并且在铜绿假单胞菌的细胞膜中也有发现。这些发现表明，巯氧吡啶类杀菌剂在细菌细胞内以及细胞膜处均具有活性。
