耐低温和不耐低温的酿酒酵母菌株之间杂种的电泳图谱。

Electrophoretic profile of hybrids between cryotolerant and non-cryotolerant Saccharomyces strains.

作者信息

Giudici P, Caggia C, Pulvirenti A, Zambonelli C, Rainieri S

机构信息

Istituto di Industrie Agrarie, University of Catania, Reggio Emilia, Italy.

出版信息

Lett Appl Microbiol. 1998 Jul;27(1):31-4. doi: 10.1046/j.1472-765x.1998.00377.x.

DOI:10.1046/j.1472-765x.1998.00377.x

PMID:9722994

Abstract

The chromosomal DNAs of cryotolerant Saccharomyces bayanus, non-cryotolerant Saccharomyces cerevisiae strains and their intra and interspecific hybrids were separated by pulsed field electrophoresis (PFGE). The cryotolerant and non-cryotolerant strains gave distinctly different electrophoretic profiles. The hybrids cryotolerant x cryotolerant and non-cryotolerant x non-cryotolerant were fertile and they gave the same electrophoretic karyotype as the respective parents. The cryotolerant x non-cryotolerant hybrids were sterile and gave electrophoretic karyotypes which showed both the bands the parents have in common and those they do not share.

摘要

通过脉冲场凝胶电泳（PFGE）分离了耐低温的巴氏酵母、不耐低温的酿酒酵母菌株及其种内和种间杂种的染色体DNA。耐低温和不耐低温的菌株呈现出明显不同的电泳图谱。耐低温×耐低温和不耐低温×不耐低温的杂种可育，并且它们的电泳核型与各自的亲本相同。耐低温×不耐低温的杂种不育，其电泳核型显示出亲本共有的条带和不共有的条带。