van Ree R, van Leeuwen W A, Aalberse R C
CLB-Sanquin Blood Supply Foundation, and the Laboratory for Experimental and Clinical Immunology, Academic Medical Centre, University of Amsterdam, The Netherlands.
J Allergy Clin Immunol. 1998 Aug;102(2):184-90. doi: 10.1016/s0091-6749(98)70084-3.
Current diagnostics for grass pollen allergy are composed of mixtures of pollen of different grass species. Their complex composition hampers accurate standardization.
The aim of the study was to investigate whether mixtures of grass pollen extracts can be replaced by a single pollen species and whether a single pollen species can be replaced by a limited number of purified natural or recombinant major allergens.
Sera (n = 800) were selected on the basis of a general suspicion for inhalant allergy and tested in a RAST for IgE reactivity with pollen from 17 different grass species. Cross-reactivity of IgE responses was studied by means of RAST inhibition. Sera with positive test results for grass pollen were tested in a RAST for natural Lol p 1 and Lol p 5 and recombinant Phl p 1 and Phl p 5.
Specific IgE antibodies against one or more of the 17 pollen species were detected in 209 of 800 sera (26.1%). The highest responses were observed against Poa pratensis followed by Festuca rubra, Phleum pratense, and Dactylis glomerata. IgE responses were clearly lower (approximately by a factor of 5) against only three species (Phragmites communis, Cynodon dactylon, and Zea mays). With the exception of a few low-responder sera, no sera were found to have negative test results to the high responder species and positive results to any of the other species. Sera with positive test results for grass pollen (n = 154) were tested with purified Lol p 1 and Lol p 5. IgE anti-Lol p 1 and Lol p 5 accounted for an average of 81% +/- 7% of total anti-grass pollen IgE. For 14 sera (all with low anti-grass pollen IgE titers), a RAST with purified allergens resulted in a false-negative diagnosis for grass pollen allergy. With recombinant Phl p 1 and Phl p 5, the mean IgE reactivity was 57% +/- 6% of the anti-grass pollen IgE response (n = 141), with 13 false-negative results.
One grass species is sufficient for in vitro diagnosis of grass pollen allergy. With purified natural Lol p 1 and Lol p 5, greater than 90% of grass-positive sera is detected. Around 80% of the IgE response to grass pollen is directed to these major allergens. Recombinant allergens, produced in Escherichia coli, did not equal the IgE-binding capacity of their natural counterparts.
目前用于草花粉过敏的诊断方法是由不同草种的花粉混合物组成。其复杂的成分阻碍了准确的标准化。
本研究的目的是调查草花粉提取物混合物是否可以被单一花粉物种替代，以及单一花粉物种是否可以被有限数量的纯化天然或重组主要变应原替代。
基于对吸入性过敏的普遍怀疑选择血清（n = 800），并在放射变应原吸附试验（RAST）中检测其与17种不同草种花粉的IgE反应性。通过RAST抑制研究IgE反应的交叉反应性。对草花粉检测结果呈阳性的血清在RAST中检测天然黑麦草Lol p 1和Lol p 5以及重组早熟禾Phl p 1和Phl p 5。
在800份血清中的209份（26.1%）中检测到针对17种花粉物种中一种或多种的特异性IgE抗体。对草地早熟禾的反应最高，其次是紫羊茅、梯牧草和鸭茅。对仅三种物种（芦苇、狗牙根和玉米）的IgE反应明显较低（约为五分之一）。除少数低反应血清外，未发现对高反应物种检测结果为阴性而对任何其他物种检测结果为阳性的血清。对草花粉检测结果呈阳性的血清（n = 154）用纯化的Lol p 1和Lol p 5进行检测。IgE抗Lol p 1和Lol p 5平均占总抗草花粉IgE的81%±7%。对于14份血清（均具有低抗草花粉IgE滴度），用纯化变应原进行的RAST导致草花粉过敏的假阴性诊断。对于重组Phl p 1和Phl p 5，平均IgE反应性为抗草花粉IgE反应的57%±6%（n = 141），有13例假阴性结果。
一种草种足以用于草花粉过敏的体外诊断。使用纯化的天然Lol p 1和Lol p 5，可检测到超过90%的草花粉阳性血清。对草花粉的IgE反应中约80%针对这些主要变应原。在大肠杆菌中产生的重组变应原，其IgE结合能力不如天然对应物。
