Wang W
Department of Physiology and Biophysics, University of Nebraska College of Medicine, Omaha, Nebraska 68198-4575, USA.
Am J Physiol. 1998 Sep;275(3):H783-8. doi: 10.1152/ajpheart.1998.275.3.H783.
I have shown that cardiac sympathetic afferent stimulation by epicardial application of bradykinin (BK) was significantly enhanced in pacing-induced heart failure (HF) dogs. This enhancement appeared to be mediated by prostaglandins. The present study was to determine whether nitric oxide is involved in this enhancement. Under alpha-chloralose (100 mg/kg iv) anesthesia, the renal sympathetic nerve activity (RSNA) response to BK was determined in 15 HF and 15 sham dogs in the sinoaortic-denervated and vagotomized state. The RSNA response to BK was significantly enhanced in HF. This enhanced RSNA response to BK was significantly reduced in the HF dogs after administration of the cycloxygenase inhibitor indomethacin (5 mg/kg iv), but no significant change was found in the sham group. In contrast, RSNA responses to BK were significantly reduced in the sham dogs after administration of the nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME, 30 mg/kg iv), but no significant change was found in the HF group. These data suggest that the RSNA response to BK is mediated by nitric oxide to a large degree in the normal state but is primarily mediated by prostaglandins in the HF state.
我已经表明，在心外膜应用缓激肽（BK）刺激心脏交感传入神经时，起搏诱导的心力衰竭（HF）犬的这种刺激显著增强。这种增强似乎是由前列腺素介导的。本研究旨在确定一氧化氮是否参与这种增强作用。在α-氯醛糖（100mg/kg静脉注射）麻醉下，在去窦主动脉神经和迷走神经切断状态下，测定了15只HF犬和15只假手术犬对BK的肾交感神经活动（RSNA）反应。HF犬对BK的RSNA反应显著增强。给予环氧化酶抑制剂吲哚美辛（5mg/kg静脉注射）后，HF犬对BK增强的RSNA反应显著降低，但假手术组未发现显著变化。相反，给予一氧化氮合酶抑制剂NG-硝基-L-精氨酸甲酯（L-NAME，30mg/kg静脉注射）后，假手术犬对BK的RSNA反应显著降低，但HF组未发现显著变化。这些数据表明，在正常状态下，对BK的RSNA反应在很大程度上由一氧化氮介导，但在HF状态下主要由前列腺素介导。
